Synthesis And Characterization Of Tetra-sulfonic Metal Phthalocyanines And Their Interaction With Bovine Serum Albumin

In this paper , the synthesis,molecular structure,characteraction of metal phthalocynines were commented. Some major analytic methods used in the study of interaction between the drug molecular with bovine serum albumin(BSA) were reviewed.Tetra-sulfonic phthalocyanine aluminium, cobalt, manganese(AlPcS₄, CoPcS₄, MnPcS₄) was synthesized by using 4-sulfonic-phthalic acid as fragment. Their structures were identified and characterized by IR, UV-VIS, TG , HPLC and ¹HNMR. The dimerization of AlPcS₄, CoPcS₄, MnPcS₄ were studied in different solution (DMSCO, H₂O, CH₃CN),different pH and different ion concentration by spectra photometic method.The interaction of AlPcS₄, CoPcS₄, MnPcS₄ with bovine serum albumin(BSA) was studied .The binding constant K_A of three metal phthalocyanine were determined by both UV/Vis and fluorescence spectra method, the same results were obtained.The binding site of tetrasulfonated metal phathalocyanine on the BSA were determined by using Hemin chloride(HE), Ibuprofen(IB)and L-tryptophan(TRP) as probes and competitive binding method. According to the Foster dipole-dipole energy transfer, the distance to be measured between the MPcS₄ and BSA are about 2-3nm.Tetra chlorosulfonyl phthalocyanine aluminium [AlPc(SO₂Cl)₄] and Tetra-carboxyl -aminohexanoic-sulfonyl phthalocyanine aluminium [AlPc(SO₂C₅H₁₀COONa)₄] were synthesized by tetra-sulfonated phathalocyanine aluminium. Their structures were identified and characterized by IR, UV-VIS, TG and HPLC.