The Studys Of Loss Of Heterozygosity,mRNA & Protein Expression Of P57～(kip2) In Human Hepatocarcinoma

Objective: To study the loss of heterozygosity (LOH) of p57^kip2 and expressions of p57^kip2 mRNA & protein in various stages of human hepatocellular carcinoma (HCC) in order to explore the molecular mechanism of hepatocarcinoma.Methods: LOH of two microsatellite loci (TH01 and D11S2359) were detected in the p57^kip2 gene site by using PCR-polyacrylamide gel electrophoresis-silver staining method. Immunohistochemistry staining and in situ hybirdization (ISH) were also used to show the expression of p57^kip2 mRNA and protein in normal liver tissue, pericancerous cirrhosis and HCC.Results: 1. LOH: LOH was identifided at least in 9 cases out of 30 (30.0%) on one microsatellite loci. The positive rates of LOH in high differentiated HCC tissues and low differentiated HCC tissues were 3.33% and 26.67% respectively, there was significant difference and positive correlation between them. 2.Expression of p57^kip2 mRNA: There were no expressions in normal liver tissue. Expressions in pericancerous cirrhosis and hepatocellular carcinoma were 33.33% and 16.67% respectively. In group of hepatocellular carcinoma tissues, the expressions in high differentiated HCC tissues and low differentiated HCC tissues were 28.57% and 0 respectively, there was significant difference and positive correlation between them.