Study On Relationship Between MTHFR Gene Polymorphism And Ischemic Cerebral Vascular Diseases

In the last decade, many studies have shown that homocysteine (Hcy) concentrations is related with cerebral arteriosclerosis. The homocystenine promotes the oxidation of low-density lipoprotein, which causes injury of vascular endothelial cells and leads to endothelial dysfunction. Homocystenine also promotes vascular smooth muscle cell growth, and increases thromboxand A2 production, platelet aggregation and factor V activity. Moderate and intermediate hyperhomoxysteinemia is believed to be associated with BI. Methylenetetrahydrofolate reductase (MTHF) is the key enzyme in the metabolism of Hcy. The catalytic domain of this enzyme is believed to locate in the N-terminal. C677T polymorphism, located in the N- terminal of MTHFR, is believed to be the most common genetic defect resulting in the hyperhomoxysteinemia. More and more researchers have paid attention to the correlation of MTHFR gene mutation and HHcy to BI etiology. This research investigated relationship of these three factors and elucidated their relevance with clinical and basic methods.Methods: MTHFR(N) coding region was amplified with PCR. PCR product was cloned into T easy plasmid and sequenced. The MTHFR polymorphisms of 50 patients and 50 normal individual from Changchun were studied by Amplification refractory mutation...