Reaserch Of Methylenetetrahy Drofolate Reductase A1298C Gene Polymorphisms And C677T、A1298C Gene Polymorphisms Relate To MTX Induce Side Effects Inuyghur And Han Nationality Relationship

Object： To study the genetic polymorphisms of methylenetrahydrofolatereductase(MTHFR) among the Uygur and Han populations in xinjiang；to investigate thepossible association of methylene tetrahydrofolate reductase (MTHFR) genepolymorphisms with adverse effects related to methotrexate (MTX)；Methods:（1）radomly collect300healthy people blood sample in People’s Hospital of Xinjiang UygurAutonomous Region；collect31patients blood sample during2010and2011.(2)Thetechnique of polymerase chain reaction and restriction fragment lengthpolymorphism(PCR-RFLP)was usded to detect the genotype and allele frequencies at1298mononucleotide sites of MTHFR among the Uygur and Han populations inXinjiang.(3) Data statistic by the software SPSS17.0statistics. Results:(1)At the site of1298,the C allele frenquencies were found to be12%and25%, for the Uygur and the Hanpopulations respectively, P<0.05, which had statistical differences.(2)The C allelefrequencies at1298mononucleotide sites of MTHFR among the Uygur in Xinjiang hadstatistical differences with those hitherto reported Mao、 Buyi populations inGuizhou.(3)The analysis highlighted a significant association of C677T polymorphismwith MTX toxicity druing Han population.(4)there are no statistically association withoverall MTX toxicity and polymorphism in Uygur patients was detected. Conclusion:(1)The polymorphisms of the1298mononucleotide sites of MTHFR were diverse indifferent populations.(2)The C allele frequencies at the site of MTHFR1298of the Uygurand Han in xinjiang was differneret. The C allele frequencies at1298mononucleotide sitesof MTHFR among the Uygur in xinjiang was different with Mao、Buyi populations inGuizhou because of different nations.(3)These results indicate the association of1298polymorphisms maybe a reduced risk of side effects of MTX in Han Population.