The Experimental Study Of Effect Of TFE On Isolated Working Rat Heart And Cardiomyocytes Ion Channels

Herba Epimdii(HE) is a kind of perennial herbage in Berberi-dacceae, also called xianlingpi in China. It is a chinese traditional for tonifying the kindney that has been used for over a thouand years.The whole grass is used as medicine,short horned Epimedium is hard,warm,nonpoisonous according to Compendium of Materia Medic.It has the effect of benefiting kidney, harding muscles and boneses, benefiting waist , knee,and mentality. Modem pharmacology prove that the major composition of HE is total flavonoids of Epimedium(TFE).TFE can suppress the growing of microorganism strengthen the immunity,expand coronary artery and postpon ageing and promote bone cell growing.Since 1990s,the pharmacology effect of TFE in cardiovascular system has been reconstred by the scholar. It has been proved TFE can decrease the heart rate, myocardial oxygen consumption, coronary arterial resistance. Moreover TFE could ameliorate the index of hemodynamics, decrease the raised myocardial enzyme. In order to investigate the direct function of TFE on cardiocytes ,we observe the effect of isolate working rat heart , action potential , L-Ica of cardiomyocytes ,and oxidative injury of cultured neonatal cardiomyocytes to study the protective mechanisms of TFE in cardiocytes,and provide theoretical reason for the clinical.Langendorff isolate working rat heart methods were used to observe the effect of different does of TFE on cardiac hemodynamics in isolated working rat heart.The heart worked by 37℃Krebs-Heseleif persistent perfusion..To observe effects of hearts were perfused at an 1.17kpa left atrial filling pressure and an 5.38kpa aortic afterload with k-H of 37.Recording the direct effects of TFE on...