Partial Quality Standard Of N-V Protease Preparation

Cardial and cerebral vascular thrombosis disease is a threat against human's health in modern time, it will lead to sudden death and nerves functional disturbance. The principal method of clinical treatment is to cut down fibrinogen in the blood and to dissolve internal clot at earlier period as soon as possible. It is the important topic to study and research this kind of drug in the medicine domain. N-V protease is a kind of protease abstracted from some marine life, N-V protease have the function of hydrolyzing plasminogen and dissolving internal clot. Using high performance liquid chromatography method to detect protease, and using high performance thin-layer chromatography method to detect the related substances NTX.1 Reverse phase high performance liquid chromatography (RP-HPLC)Use the Agilent poroshell 300SB-C18 (21×75mm) which suited for reversed-phase separation of protein and other biomacromolecules produce by the Agilent company recently. The retention time of the main peak is 0.42min and the response value is 0.020AU. The retention time is short他that is concern with the length of the column is 75mm.This columns length is half of common. It is also have been find out that, retention time did not alter obviously with the increasing of organic phase in the mobile phase, we can conclude that the main peak is not a protein peak. The elution time is...