Study Of Porous Calcium Phosphate Cement And The Composite Of Dan-Shen/Calcium Phosphate Cement

The calcium phosphate self-setting cements (CPC) have many advantages, such as, the excellent biocompatibility, self-setting under ambient conditions, and almost without heat release. Therefore it has attained more attentions and been applied in clinic to repair and replace the bone defects. However, CPC lack of the macropores ranged from 100um to 500um, which are required for new bone ingrowth. Meanwhile, drug and growth factor are necessary to treat the bone disease or to promote the bone formation in osteopathies. Therefore, many research focused both on the preparing porous CPC and the applying CPC as a carrier of drugs and growth factors. Our attempts are to prepare porogens-contained CPC which can self-emerge macropores with the suitable porosity and pore size in vivo. The other aim of the present study is to investigate a Chinese medicine with osteogenesis activity. Then it was incorporated into CPC. The biocompatibility of the CPC was evaluated by in vitro cell culture assays.In this study, the formulation of calcium phosphate cements coincided with that of Biocement D. The cement powder was comprised by mixing a-Tricalcium phosphate (α-TCP, α-Ca₃(PO₄)₂), Dicalcium phosphate dehydrate (DCPD, CaHPO₄·2H₂O), Sintered hydroxyapatite (HA, Ca₅(PO₄)₃OH) and calcium carbonate (CaCO₃) in the ratio of 58/25/8.5/8.5 (w/w/w/w). The liquid phase was Na₂HPO₄and NaH₂PO₄ solution. Five kinds of porogens, including poly-lactic-acid (PLA) microsphere, gelatin microsphere, soudium chloride (NaCl), glucose and mannitol, with the desired particle size, were prepared or chosen. The porogen-incorporated CPC were prepared by mixing each porogen with the cement powder and liuquid phase. Then they were immersed in the simulated body fluid (SBF) to form macropores CPC. The results show that PLA microsphere could be degraded to form...