The Study On The Effect Of Teprenone On Steroids-induced Gastric Mucosal Damage

Objective To estimate the gastric mucosal protective effect of teprenone administration on steroids-induced gastric mucosal damage.Methods Fifty male Sprague-Dawley rats (230-250g) were divided into normal control group, model control group, and model therapy groups (teprenone 50 mg/kg,100 mg/kg, 200 mg/kg). Rats were fasted for four days, and during which time, they were injected prednisolone at doses of 40 mg/kg subcutaneously. Model therapy groups were treated by teprenone at doses of 50 mg/kg, 100mg/kg and 200mg/kg, for 7 days respectively. Gastric ulcer index and the grade of histological lesions were measured. ET-1 level in blood plasma and the mucosal PGE₂ level were determined by radioimmunoassay. NO level in blood was determined by Griess method. The mRNA expression of HSP70 in the gastric tissue was semi-quantitatively detected with reverse transcription-polymerase chain reaction. The protein expression of c-fos, iNOS in the gastric tissue was detected with Immunohistochemical staining.Results In model control group, the ulcer index, grade of histological lesions and ET-1 level increased significantly compared with normal controle group(44.5 vs 0, 5.5 vs 0, and 399 pg/ml±74 pg/ml vs 279 pg/ml±56 pg/ml, all P < 0.01), PGE2 level decreased significantly (154 pg/mg±83 pg/mg vs 337 pg/mg±112 pg/mg , P < 0.01) , NO level has no significant difference with normal control group, the expression of c-fos, iNOS were stronger than that in normal control group (all P < 0.01), and the expression of HSP70mRNA was...