The Mechanism Of Icariin-induced Vasodilation Of Canine Isolated Coronary Artery And Its Protective Effects On Endothelial Cells

Objective: To investigate the mechanism of icarin-induced vasodilation of canine isolated coronary artery and protective effects of icariin on endothelial cells (EA. hy926 cells).Methods: The isolated coronary artery was removed from the beagle dogs and cut into rings 5mm in length. The coronary rings were divided into some groups. the rings were mounted in an organ chamber and contracted by either 40 mM KC1 or 10 mM PGF2α, then observed the effects of icariin and some related inhibitors on the precontracted coronary arterial rings. After the organ bath, the rings were quickly removed to the liquid nitric and examined the eNOS gene. EA. hy926 cells were cultured in vitro and added quercetin and icariin of different concentration into the endothelium, then measured the production of NO, and examined the expression of eNOS protein and gene at different time.Results: Icariin can relax the coronary arterial rings in a concentration-dependent manner (P<0.05), the vasorelaxant effect of icariin was blocked by L-NAME (eNOS inhibitor), ODQ (GC inhibitor) but not by indomethacin (COX inhibitor) and glibenclamide (Katp...