Experimental Study On Mechanism Of Effect Of Hepatocyte Transplantation

Objective: To establish an experimental model that hepatocyte transplantation treats the acute liver failure of rats induced by D-Galactosamine, prove the hypothesis of an important effect which the proliferation and differentiation of hepatic oval cells have exerted on the liver regeneration after rat liver has been impaired, and expore the mechanism that hepatocyte transplantation reverses the acute liver failure caused by D-Galactosamine.Methods: Hepatocyte transplantation was carried out intraperitoneally after the acute liver failure of rats had been induced by D-Galactosamine. The entire experiment included PartⅠand PartⅡ. In PartⅠ,these rats were injected by D-Galactosamine(19.5g/kg) and immediately divided randomly into Group A and Group B. 48 hours after intoxication, 4×107 allogeneic hepatocytes were transplanted intraperitoneally into every rat in Group A ,while 10 ml of normal saline were injected intraperitoneally as control in Group B. Then the survival rate for two weeks was assessed. In PartⅡ,after the liver function index,liver histology and AFP of the normal rats were observed, these remnant rats were induced to acute liver failure by D-Galactosamine and were divided randomly into Group C and Group D. Then these two groups were transplanted by the same methods as those in Group A and Group B respectively. The liver function index, the AFP values and liver histology of rats in Group C and D have been examined by radioimmunoassay and histological technologyResults: 1,Survival rate of group A was higher than that of group B(63.16% vs 15.00%, P﹤0.05).2,There was significant difference in AFP value between Group C and Group D, and significant difference also lies in the groups before and after transplantation (P﹤0.05).3,There was no significant difference in liver function index between Group C and Group D(P﹥0.05), and between the groups before and after transplantation (P﹥0.05).4,Liver histology of Group C was more improved than that of Group D.There was significant difference in hepatic oval cell numbers between Group C and Group D, and significant difference also lies in the groups before and after transplantation (P﹤0.05).Conclusion:1,Survival rate and the liver histology of rats suffering from acute liver failure induced by D-Galactosamine were improved via intraperitoneal hepatocyte transplantation.2,Hepatic oval cells of non-activation and activation after liver failure could be stimulated by these transplanted hepatocytes and their proliferation and differentiation could be speeded.They could exert an important effect on the reversion of liver failure.