The Significance Of Expression Of Minichromosome Maintenance 5 Gene In Ovarian Epithelial Tumor

Objective: To explore the expression * and significance of minichromosome maintenance 5 (MCM5) gene in ovarian epithelial tumors.Methods: Reverse transcription polymerase chain reaction (RT-PCR) and immunocyto/histochemistry were applied respectively to examine the mRNA and protein of MCM5 in SKOV-3 cell line, normal ovarian tissue (15 cases), benign (12 cases) and malignant (40 cases) ovarian epithelial tumor tissues. Electrochemiluminescence immunoassay (ECLIA) was used to test CA125 in serum of all 67 cases.Results: (1) Overexpression of MCM5 was detected in SKOV-3 cells and the expression of MCM5 was significantly up-regulated in carcinomas than that in normal or benign tumor tissues (P<0.05). (2) There was positive correlation between expression of MCM5 mRNA and protein with significant Pearson coefficient of correlation (P < 0.01). (3) There was no significant difference in MCM5 expression between serous and mucinous tumors (P>0.05), and no association with age (P>0.05). The frequency of MCM5 expression in ovarian epithelial carcinoma showed a tendency to increase as histological grade increasing and there was significant difference in MCM5 expression among G₁,G₂ and G₃ tumor (P<0.05). (4) The expression of MCM5 in ovarian tumor tissues showed a positive correlation with the level of CA125 in serum (P<0.01).Conclusion: MCM5 may play an important role in the genesis and progression of ovarian epithelial tumor. It may become a new marker of proliferation of malignant cells used in diagnosis and prognosis, and a possible target of gene therapy for ovarian epithelial tumor.