Expression Of PTTG And Its Relation With The Expression Of P53,c-myc,cyclinB1 Protein In Epithelial Ovarian Cancer

Objective It has been suggested that pituitary tumor-transforming gene (PTTG), a novel oncogene, has played an important role in many human malignancies. However, little is known about its precise role in genesis and development of epithelial ovarian cancer. In this study , the expressions of mRNA and protein of PTTG were detected and the relationship with the clinical-pathological features, the expression of p53, c-myc, cyclinB1 protein were analyzed in epithelial ovarian cancer.Methods Expression of PTTG mRNA and protein were assessed by reverse : transcription polymerase chain reaction and immunohistochemistry method respectively. in 61 cases of epithelial ovarian cancer and compared with clinical characteristics, such as clinical-surgical stage,and expression of p53,c-myc, cyclinB1 protein. The corresponding benign and normal ovarian tissues were investigated as control group.Results 1. PTTG mRNA can be detected in all of the three tissues. PTTG mRNA was expressed in 96.7% cases of epithelial ovarian cancers and 72.4%cases of benign epithelial ovarian tumors and 33.3% cases of normal ovarian tissues. The average expression quantity of PTTG mRNA is 0.884±0.108 in epithelial ovarian cancers, 0.655±0.180 in benign epithelial ovarian tumors and 0.345±0.130 in normal ovarian tissues respectively. The expression rate and average quantity of PTTG mRNA is significantly higher in epithelial ovarian cancers than in benign epithelial ovarian tumors as well as in normal ovarian tissues (P<0.01). 2. The average quantity of PTTG mRNA was higher in stage Ⅲ and Ⅳ than that in stage Ⅰ and Ⅱ (0.910±0.078 VS 0.811±0.145, P=0.00731). Similarly, the average quantity of PTTG mRNA was significantly higher in tissue with lymphatic metastasis than in those without lymphatic metastasis (0.927:10.096 VS 0.850±0.106, p=0.02152) . There was no relationship between PTTG mRNA expression and age, pathological type and histological grade (P>0.05). 3. PTTG protein was expressed in 66.7% cases of epithelial ovarian cancers and 33.3% in benign epithelial ovarian tumors, but no expression of PTTG protein was found in normal ovarian tissues. The expression of PTTG protein in epithelial ovarian cancers is significantly higher than in benign epithelial ovarian tumors and normal ovarian tissues (P<0.01). 4. The expression rate of PTTG protein was stronger in stage Ⅲ and Ⅳ 80.0% than in stage Ⅰ and Ⅱ 33.3% (p=0.01121). Similarly, the expression rate of PTTG protein was stronger in tissue with lymphatic metastasis 83.3% than in those without lymphatic metastasis 54.2 % (p=0.09821). There was no relationship between PTTG protein expression and age,...