Primary Gynecological Malignancies P15mrna Study Of Abnormal P15 Protein Expression

Objective To study the aberrant expressions of p15 mRNA and protein in primary gynecologic malignancy, and to define the role of p15 gene in the generation and development of primary gynecologic malignancy. Methods By using of reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry assays, expressions of p15 mRNA and protein were detected in 48 primary epithelial ovarian carcinomas, 42 primary endometrial carcinomas, 40 primary cervical carcinomas, and the corresponding normal tissues. Results ①The loss of expressions of p15 mRNA and p15 protein were found to be 11 (22.92%) and 13 (27.08%) in 48 primary epithelial ovarian carcinomas, respectively, 16 (38.10%) and 18 (42.86%) in 42 primary endometrial carcinomas, respectively, and 16 (40.00%) and 17 (42.50%) in 40 primary cervical carcinomas, respectively. Expressions of p15 mRNA and p15 protein were detected in all of the corresponding normal tissues. ②There was a correlation between the expressions of p15 mRNA and protein and histological types in primary epithelial ovarian carcinomas (p<0.05), and although their expressions were irrelevant to FIGO stages and histological grade, there was a tendency for them to increase with the stage and grade increasing. ③The expressions of p15 mRNA and protein were irrelevant to histological types, FIGO stages and histological grade in primary endometrial carcinomas and in primary cervical carcinomas, but there was a tendency for them to increase with the stage and grade increasing. Conclusions The aberrant expressions of p15 mRNA and protein suggest that it is associated with tumorigenesis in primary gynecologic malignancy.