1.Effect Of 2-Methoxyestradiol On Proliferation And Apoptosis Of Rat' Neurons And C6 Glioma Cells 2.Study The Protective Effects Of Low-Frequency Pulsed Electromagntic Field On Hippocampal Neurons Following Cerebralischemia Injury In Rats

PART ONEEFFECT OF 2-METHOXYESTRADIOL ON PROLIFERATION AND APOPTOSIS OF RAT' NEURONS AND C6 GLIOMA CELLSObjective To investigate the effects of 2-methoxyestradiol to the growth inhibition and apoptosis on C6 glioma cells and neuron cells in vitro. To provide certain theory basis for the treatment of glioma.Methods The frozen C6 cells were resuscitated and cultured in DMEM mediums supplemented with 10% bovine calf serum, 1 × 10U/L penicillin and 100mg/L streptomycin to logarithmic phase in CO₂ culturing box. To obtain cerebral cortex from fifty days old Wistar rat. Neurons were cultured in CO₂ culturing box. The C6 glioma cells and neurons were divided into control group and four 2-methoxyestradiol treatment groups according different doses of 2-methoxyestradiol.The C6 glioma cells and neurons were treated by different doses of 2-methoxyestradiol at different times. They were observed under the microscope and electromicroscope. The viability was examined by MTT assay, cell-cycle and apoptosis were examined by flow cytometry.Results 2-methoxyestradiol caused a dose and time-dependent inhibition of the C6 cell viability(P<0.05). 2-methoxyestradiol could induce a dose and time-dependent apoptosis of C6 cells. After treating with 2-methoxyestradiol, the number of C6 cells in the G₁ and S phase decreased while that of G₂ phase increased (P<0.05). 2-methoxyestradiol has no effect on neurons.Conclusions 2-methoxyestradiol can inhibit the proliferation and induce the apoptosis of C6 glioma cells, but has no effect on neurons. PART TWO STUDY THE PROTECTIVE EFFECTS OF LOW-FREQUENCYPULSED ELECTROMAGNTIC FIELD ON HIPPOCAMPAL NEURONS FOLLOWING CEREBRALISCHEMIA INJURY INRATSObjective To investigate the protective effects of low-frequency pulsed electromagnetic field (LFPMF) on hippocampal neurons following cerebral ischemia injury in rats.Methods 24 adult male Sprague-Dawley rats were randomly divided into radiation group (n=12) and control group (n=12). The global ischemia models of rats were produced by modified Pulsineli method. Rats in treatment group were exposed to 20 mT intensity LFPMF of 10Hz for 45 minutes per day for 4 days. The electroencephalogram(EEG) was recorded by powerlab system. Nissl staining was used to perform quantitative analysis of neuron number and immunohistochemistry staining provided the information of the distribution of positive cells to caspase-3.Results The amplitude and frequency of the EEG were higher in the radiation group than those in the control group (P<0.05). The number of neurons in the hippocampal CA1 area in the radiation group was significantly higher than that in the control group (P<0.05). The positive rate of caspase-3 in the hippocampal CA1 area in the radiation group was lower than that in the control group (P<0.05)Conclusions The LFPMF (20 mT, 10Hz) treatment has neuroprotective effect in the rats with global cerebral ischemia injury through restraining the neuron apoptosis.