| Background In clinical practice, infertility affects approximately 15 % of couples trying to conceive and a male cause is believed to be a sole or contributing factor in approximately 40% of these cases. Male infertility is complicated male syndrome. Detection of semen routine is the most important item of male infertility. With the development biochemistry, detection of seminal plasma biochemical marker quickly develops. Seminal plasma biochemical marker might reflect the function of affiliated sex gland.Detection of seminal plasma biochemical marker can help to develop new standard for clinical diagnosis, so to evaluate secretary function of male affiliated sex gland and male fertility. Semen routine analysis still is an important marker of diagnosing male infertility. How to incorporate semen routine analysis and seminal plasma biochemical marker has been an essentially solved question. To solve the question can make clinical doctor improve diagnostic procedure, shorten diagnostic cycle of patients, avoid blind examination, decrease unnecessary expenditure. This provides experimental evidence for function of affiliated sex gland in oligozoospermia, asthenozoospermia, and teratozoospermia.Objective This text studies that seminal plasma biochemical marker evaluate male infertility and relationship between seminal plasma biochemical marker and seminal parameters. This study includes the relationship of biochemical marker epididymis, seminal vesicle, and prostate between infertile group and fertile group, and the influence of sexual abstinence time and seasonal variation on seminal plasma biochemical marker. In order to incorporate semen routine analysis and seminal plasma biochemical marker, this paper study relationship between seminal plasma fructose, neutral alpha-glycosidase, carnutine, seminal plasma zinc, acid phosphatase, citric acid, PSA, seminal plasma NO and spermdensity, sperm motility, sperm morphology, et al. The purpose provides experimental evidence for improving diagnostic procedure of male infertility.Method Sperm samples were obtained from 684 patients of male infertility. The sperm morphology and sperm vitality analysis using semiautomatic sperm morphology analyzer was performed. Semen viscidity and pH were detected after semen liquefied. Seminal activity of fructose concentration, neutral alpha-glucosidase, seminal plasma zinc and acid phosphatase were measured by spectrophotometry. The concentration of carnutine was detected by improving DTNB. Enzyme-linked immunoadsorbent assay was used to determine the concentration of PSA. They were detected by corresponding kit.Result①Seminal plasma fructose concentration, neutral alpha-glucosidase, carnutine, seminal plasma zinc, acid phosphatase, citric acid, concentration of PSA of infertile group was significantly lower than that of fertile group(P<0.05). Seminal plasma NO of infertile group was significantly higher than that of fertile group(P<0.05).②Seminal plasma fructose concentration of sexual abstinence >or =6days group was significantly lower than that of sexual abstinence 1-3day group(P<0.05).However, neutral alpha-glucosidase, carnutine, seminal plasma zinc was significantly higher than that of sexual abstinence 1-3day group(P<0.05). Neutral alpha-glucosidase activity of 12-2Month was significantly lower than that of 3-5Month, 6-8Month, 9-11Month group (P<0.05, P<0.01).③Seminal plasma biochemical marker of abnormal semen content and abnormal pH group were no statistics difference in comparison with corresponding normal group (P>0.05). Seminal plasma zinc and concentration of PSA of abnormal viscidity group was significantly lower than that of normal viscidity group(P<0.05).④There was a significantly negative relative between sperm concentration and seminal plasma fructose concentration(r =–0.348, P<0.01). There was a significantly positive relative between sperm concentration and seminal plasmaneutral alpha-glucosidase, seminal plasma zinc(r =0.246, P<0.001; r =0.374, P<0.05). There was a significantly negative relative between sperm vitality and seminal plasma fructose concentration (r=–0.185, P<0.05). There was a significantly positive relative between sperm vitality and seminal plasma neutral alpha-glucosidase, carnitine (r =0.220, P<0.01; r =0.326, P<0.05). There was a significantly positive relative between sperm morphology and seminal plasma neutral alpha-glucosidase (r =0.383, P<0.05).⑤Seminal plasma fructose concentration of abnormal concentration was significantly higher than that of normal concentration and fertile group(P<0.05). Seminal plasma fructose concentration of abnormal vitality group were no statistics difference in comparison with fertile group (P>0.05). Seminal plasma neutral alpha-glucosidase of abnormal density, vitality, morphology was significantly lower than that of corresponding normal group and fertile group(P<0.05). The carnitine concentration of abnormal density, vitality, morphology group was significantly lower than that of fertile group(P<0.05). The carnitine concentration of abnormal vitality group was significantly lower than that of normal vitality group(P<0.05). Seminal plasma zinc of abnormal density group was significantly lower than that of corresponding normal and fertile group (P<0.01). Seminal plasma acid phosphatase of abnormal vitality, morphology group was significantly lower than that of corresponding normal group(P<0.05). Seminal plasma citric acid of abnormal vitality group was significantly lower than that of fertile group(P<0.05). Seminal plasma PSA of abnormal density, vitality group was significantly lower than that of corresponding normal and fertile group(P<0.05). Seminal plasma NO of abnormal morphology, vitality group was significantly higher than that of corresponding normal and fertile group(P<0.05);Conclusion 1. Seminal plasma biochemical marker might evaluate male infertility.①Seminal plasma neutral alpha-glucosidase and carnitine of infertilemale significantly decrease.②Seminal plasma fructose concentration of infertile male(no azoospermatism) significantly decrease..③The biochemical marker of prostatae(seminal plasma zinc, acid phosphatase, citric acid, PSA) all significantly decrease in comparison with fertile group.④High NO concentration of seminal plasma influences on male infertility.⑤Sexual abstinence days and seasonal variation might influence on seminal plasma biochemical marker.2. There are correlations between seminal plasma biochemical marker and semen parameters.①Semen viscidity is concerned with seminal plasma zinc and PSA.②There was a significantly negative relative between sperm density and seminal plasma fructose concentration.③There was a significantly positive relative between neutral alpha-glucosidase and sperm density, vitality, morphology.④There was a significantly positive relative between carnitine and sperm vitality.⑤Seminal plasma zinc is concerned with sperm density. Seminal plasma zinc of infertile male significantly decreases.⑥Acid phosphatase might be concerned with sperm vitality, morphology.⑦Seminal plasma citric acid of infertile male significantly decreases in comparison with fertile male.⑧There was a significantly positive relative between seminal plasma PSA and sperm density, vitality.⑨Seminal plasma NO is connected with sperm vitality, morphology. The relation of quantity-effect exists, low NO concentration is advantageous to sperm parameters and high NO concentration is harmful to sperm parameters.
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