Evaluation Of Degradation Rate And Biocompatibility Of Five Different Synthetic Scaffolds In Vivo

Objective:A study about the degradation rate and the biocompatibility of five different synthetic scaffolds include: silk fibroin /Hyaluronic acid(Hyaluronic Acid 3%, 5%, 10%) , silk fibroin/collagen, PLGA (Poly-Lactic-co-Glycolic acid) in vivo. We expect this study can help us to predict the safety and feasibility of these scaffolds in clinical use.Method: All Scaffolds were implanted subcutaneous in the New Zealand rabbit through surgical method, all Scaffolds were sterile before operation. We measure the thickness of each scaffold by microscope before and after operation. 5 rabbits were excuted in 14th day after the operation in random principle, the rest 5 rabbits were excuted in 21th day after the operation. Right after specimens were harvested. HE stain was then performed. We choose the same area of each specimen and take picture. The number of cells on the picture was count by image pro plus software.Results: (1) No rabbit is dead during the experiment, no scaffold was expelled; (2) For each kind of scaffold , the cell number and the thickness of individual residual scaffold keep changing, The data of 21th day is statisticly higer than the data of 14th day after operation (P<0.05) ;(3)Cell number and thickness change ratio of PLGA is statistically higher than other kinds of scaffolds which were harvested in the same day after the operation (P<0.05) ; (4)There is no statistic difference between A, B, C, D in those 2 index (P>0.05).