The Influence Of Different Concentration Of Taurine On Biological Effect Of Hyperhomocysteinemia In Rats

Objective: To observe the influence of taurine on biological effect of hyperhomocysteinemia in rats and to explore the possible mechanismes that taurine inhibits biological effect of hyperhomocysteinemia and controls atherosclerosis in rats.Methods: Forty male wistar rats weighing 200± 20g,were acclimatized for one week prior to being divided randomly into the following groups :1. control group(10): control group were fed a standard rat diet and regular water. 2.Hhcy group(10): Hhcy group were fed a standard rat diet supplemented with 3% methionine and regular water;3.1%Tau group(10): 1% Tau group were fed above-mentioned diet and water containing 1% taurine;4.2%Tau group(10): 2% Tau group were fed above-mentioned diet and water containing 2% taurine.Every rat was fed 10 gram diet and 20 milliliter water every day for eight weeks.At the end of the test,all of the animals were anesthetized through injecting 2% pentobarbital sodium into abdominal cavity.Blood was centrifugalized and prepared for blood plasma stored in -70℃ freezer.The following indexes were examinedrHcy, MDA, SOD,NO,ET.The tissue changes of aortas were observed by light microscope.Results: 1 .Homocysteine: At the end of the test,homocysteine of the control group is significantly lower than all the other groups among which there are no differences .It is showed that to feed rat on a standard diet supplemented with 3% methionine for eight weeks can cause hyperhomocysteinemia in rat and taurine can not reduce homocysteine concentration.2.MDA: At the end of the test,MDA of the Hhcy group is significantly higher than all the other groups among which there are no differences.It is showed that l%taurine and 2% taurine can reduce the MDA...