The Observation On Neovascularization Of Bone Marrow Serum From Normal And Vascular Intima Injured Rabbits

[Objective] The applications of autologous transplantation of bone marrow (BM) stem cells in clinic have achieved great advance. However, the mechanisms of BM stem cells improving cardiac function are in doubt. Some evidence showed that treatment of ischemic heart disease with stem cells significantly enhanced neovascularization, and stem cells-enhanced neovascularization was likely mediated by increases in endogenous vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF) and angiogenin-1(Ang-1) expression. No previous study has investigated whether there are some angiogenic growth factors in bone marrow serum.A number of studies suggest that angiogenic growth factors of blood serum dramatically increased following vascular intima injury, and this cytokines may contribute to mobilization of BM stem cells. Whether there are some difference in the concentration of angiogenic growth factors in BM serum between normal and vascular intima injured rabbit have not been demonstrated.The purpose of the present study was to determine whether normal BM serum and BM serum of rabbit suffered from vascular intima injury could promote neovascularization on the chick embryo chorioallantoic membrane (CAM) model. [Methods] New Zealand White rabbits were anesthetized with sodepent, a 2.5F balloon catheter was passed into the abdominal artery through the femoral artery. The balloon was then inflated with air (2-4 kPa) and the catheter was withdrawn for three times to damage the vascular intima. Normal blood and normal BM were...