| Background :Experiment 1 :The effects of implants on the growth of CAM were studied by using the fast growing period of CAM from day 8 to day 12.Experiment 2 :The effect of DCEF on the vascular growth of injured area was observed by adding the extracorporeal DCEF into the hypocotyls of chicken embryo and the hydroxyapatite model.Objective:Experiment 1 :HA was loaded into CAM model to establish the model of HA vascularization.Experiment 2 :The effects of stable DCEF on the prophylactic prophylaxis and osteogenesis of tissue engineered bone were observed by observing the effect of DCEF on the HA model of CAM.Methods :Experiment 1 :Twelve romantic eggs were hatched to the 8th day under the same suitable conditions and randomly divided into two groups.After the window was opened,the experimental group was implanted with hydroxyapatite material,and the hydroxyapatite model was established.The control group into the filter paper material,sealed into the constant temperature and humidity box to continue to hatch.After incubation to day 12,the images were collected by CSW-X video microscope.Image J collects the length of theCAM vessel and the data per month for the length of the vessel.And the use of tissue sections to observe the hydroxyapatite and filter paper on the blood vessels of the new situation.Experiment 2 :After opening the window,12 eggs were implanted with hydroxyapatite on the 8th day of the eggs,and the hydroxyapatite model was established.The surface of hydroxyapatite was melted by melting agar and then randomly divided into two groups.After sealing the model,move into a constant temperature and humidity box and use a silver needle to insert the agar conductive.The experimental group was loaded with-1.5v exogenous stable DCEF and incubated continuously.The control group did not load DCEF incubation.After 24,48 hours,the images were collected by CSW-X video microscope.Adobe Photoshop CS2 software handles images.Image J and AngioTool software to collect the length of blood vessels and vascular growth direction data.And the use of tissue sections to observe the hydroxyapatite material angiogenesis.Results:Experiment 1:(1)After incubation for 12 days,the average angioplasty length of the experimental group was 24.031 ± 2.735 mm,and the average angiogenesis per unit area was 0.242 ± 0.046 mm / mm2.The average angioplasty length of the control group was 23.561 ± 1.661 mm,and the average angiogenesis per unit area was 0.212 ± 0.052 mm / mm2.There was no significant difference in angiogenesis between the two groups(P> 0.05).(2)The experimental specimens of hydroxyapatite were stained with vasculartissue.Experiment 2:(1)After 24 hours of incubation in DCEF,the average length of CAM angiogenesis was 11.93 ± 0.89 mm in the experimental group and 8.69 ± 0.71 mm in the control group.After 48 hours of incubation,the average length of CAM angiogenesis was 12.66 ± 0.65 mm in the experimental group.The average length of CAM angiogenesis was 9.65 ±0.57 mm in the control group.The difference was statistically significant(P<0.05).(2)After 48 hours of incubation in DCEF,the mean blood vessel angle of the experimental group was 87.16 ° ± 54.76 ° to 23.36 ° ± 21.63 °,and the trend of vascular centripetal growth was obvious.The mean vascular angle of the control group was 81.07 ° ± 47.23 ° and changed to 83.17 ° ± 51.64 °,and the direction of angiogenesis was not changed obviously.(3)After 48 hours of incubation in DCEF,the rate of angiogenesis was 0.101 mm / h in the experimental group and 0.047 mm / h in the control group.Conclusion:Experiment 1 :The results showed that the HA model of CAM could be used as an in vitro animal model for the study of ideal bone graft material and angiogenesis drug,and HA had no effect on angiogenesis in CAM.Experiment 2 :The results showed that the loading of exogenous Stable DCEF could promote the growth of the blood vessel to the negative of the electric field. |
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