Sophora Alopecuroides Extract On Human Colon Cancer Cell Line HCT116Strains Of Chick Embryo Chorioallantoic Membrane Experimental Study On Tumor Angiogenesis

Objective：Through the establishment of the people HCT116colon cancer cell lineschicken embryos allantoic membrane transplantation tumor model, to observe xinjiangbitter beans extract huai set after transplantation of alkali role of anti-angiogenicphenomenon, and observe xinjiang bitter beans thick total alkaloid in the role of coloncancer cells HCT116people antiproliferative and induce the apoptosis of function, thispaper discusses the xinjiang bitter beans antitumor, and the mechanism of action ofangiogenesis. Method： By MTT method, flow cytometry detection of sophoraalopecuroides xinjiang was rough total alkaloid against human colon cancer cellproliferation HCT116and induce the apoptosis role, and observe chicken embryos fluffyallantoic membrane transplantation tumor angiogenesis changes, with the positive buyfluorescent microscope the ratio of blood vessels; Immunohistochemical method todetermine the chicken embryos fluffy allantoic membrane transplantation tumors VEGFdyeing strength and the change of MVD. Result：Xinjiang bitter beans thick totalalkaloid colon cancer cells to HCT116role24,48h IC50alue concentration were24mg/ml,6.82mg/ml. Xinjiang bitter beans thick total alkaloid can reduce people HCT116colon cancer cell cycle G1phase percentage, heighten S phase and can induce peopleHCT116colon cancer cell apoptosis. Bitter beans extract set in the role of CAM treealkali medicine group than blood vessels after area (VA/CAM) were less than modelcontrol group (P <0.05), and at the same time, reduce tree set of the tumor, reduce theexpression of VEGF microessel-density (P <0.05). Conclusion：Xinjiang bitter beansthick total alkaloid can restrain people HCT116colon cancer cell proliferation, and can induce the apoptosis, interfere with the cell cycle G1and S phase, which block the tumorcell mitosis. Bitter beans extract set in the role of alkali tree chicken embryos fluffyallantoic membrane transplantation tumor has obvious antiangiogenic effect, itsmechanism may reduce the expression with tumors VEGF and reduce microessel-densityrelevant.