The Protective Effects Of Catalpol On Neuron Cell Induced By Ischemia-reperfusion

The purpose of this study mainly aims at observation the effects of catalpol and its action mechanism in neuron cell ischemia-reperfusion model.PC12 cells is usually uesd as neural biology and neural chemical model. PC12 cells attacked by ischemia-reperfusion were designated as the model by which the protective effects of the catalpol on the cell damages from ischemia were investigated. Fluorescence staining and lactate dehydrogenase (LDH) assay were used to investigate the effects of catalpolon cell membrane structures. The experiment results indicated that catalpol protected PC12 cells against ischemia significantly, which may be mediated by stabilizing the cell membrane structures.In order to make clear the neuroprotective mechanism of catalpol, the activities of endogenous antioxidants and the content of lipid peroxide were assayed, mitochondrial membrane potential was measured by Microplate-reader, the protein of P53 and Bcl-2 were measured by flow cytometry analysis (FCM).The results proved that catalpol improved the GSH-Px and SOD acivities, and inhibited iNOS activity ,reduced the content of lipid peroxide and ROS, prevented the decrease in the level of Bcl-2 protein, inhibited activation of caspase-3 and the reduction of mitochondrial membrane potential. All these suggested that catalpol was a potential neuroprotective agent and its neuroprotective effects were achieved at least partly by promoting endogenous antioxidant enzymatic activities and inhibiting free radical generation and activation of caspase-3, regulation of Bcl-2 and P53.To further illmulate the effect of catalpol, cortical neurons attacked by ischemia-reperfusion were designated as the model by which the protective effects of the catalpol on the cell damages from ischemia were investigated. The activities of endogenous antioxidants and the content of lipid peroxide were assayed. The results proved that catalpol improved the GSH-Px and SOD acivities, and inhibited iNOS activity ,reduced the content of lipid peroxide.