RNA Interference Silencing Expression Of Survivin Gene And Reversing Drug Resistance Of Cervical Cancer Cells HeLa

Objective: Through interfering the expression of surviving with short hairpin RNA (shRNA) technology, to investigate the effect of down regulation of survivin on cell apoptosis and chemosensitivity to Cisplatin in cervical cancer cells Hela.Methods: Using Hela cells as a model system, three groups were set up transfected with lipofectamine, RNAi control plamid and pSSURV (survivin RNAi plasmid), respectively. The expression of survivin in Hela cells was measured at transcriptional and translational level by using RT-PCR and western-blotting methods. The viability of cells applied with different dose of DDP was determined by using the method of 3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide reduction (MTT method). The effect of the DDP on the cell cycle and apoptosis was analyzed with flow cytometry.Results: RT-PCR and western blotting demonstrated that survivin expression was significantly decreased by transfection with RNAi targeting plasmid; the expression proportion was reduced by nearly 70%. DDP significantly strengthened the chemosensitivity of the cells with a dose-dependent manner. The cell viability of RNAi vehicle group was almost equally to that of the lipofectamine control group. On the contrary, the cell viability of pSSURV group was dramatically decreased, only almost 50% of that of control with high concentration of DDP.Conclusion: These results showed that by inducing RNAi-targeting plasmid,...