| Objective: Intracerebral hemorrhage (ICH) is a common disease in nervous systerm, and it is characteristic of high disability rate and case fatality rate. Now to investigate brain tissue's damage mechanism after ICH became hot spot in the clinical and emperical study. For the past few years, animal experiment and clinical studies have all indicated that apoptosis participats brain injured after ICH, and the apoptosis is correlated to ICH's course of disease and prognosis closely. To study and research apoptotic signal conduction's rule of intracerebral hemorrhage, how morning suppress apoptotic further and to promote neurofunctional recovery, they are very important for instruction of clinical treatment. This experiment's purpose is to investigate the changes of apoptosis expression, apoptotic executant—Caspase-3 and apoptotic conductive signal Cyt c, Fas with time in lateral cerebral striatum of rats caused by intracerebral hemorrhage and the influence of early treatment with hematoma aspiration on it.Methods: 39 mature male Sprague-Dawley rats were randomly divided into normal control group, sham operation group, natural recovery group and hematoma aspiration group. The natural recovery group was divided into 6h, 12h, 24h, 3d, 7d, 11d bexakis time spots, and the hematoma aspiration group was divided into 12h, 24h, 3d, 7d, 11d pentakis time spots. ICH models was induced in rats by injection of typeⅣcollagenase into the right caudate nucleus. The control group included the normal rats; the sham operation group included those normal rats that were induced in rats by injection of saline water to substitute typeⅣcollagenase into the right caudate nucleus; those rats that were induced in by typeⅣcollagnase only but were not undertaken were belong to the natural recovery group; those rats that were aspirated 6h after collagenase injection were belong to hematoma aspiration group. The every group's animals were anesthetized. After the brain was took out, 5μm paraffin section was made according to the conventional procedure and then to make tissue's chips. By the methods of TUNEL and immunohisto- chemistry, we investigated the dynamic changes of apoptosis andexpression of caspase-3, cytochrome c and Fas.Results:1. there were a little TUNEL-positive cells in the normal control group and sham operation group. TUNEL-positive cells increased in the model group 6h after collage- nase injection, there was significant difference to compare the sham operation group, peaked at 3d and then declined, and after 11d there were still many apoptotic cells. The apoptosis of hematoma aspiration group were fewer than those of natural recovery group at 12 h, 24h and 3d, the difference was significant (P<0.05).2. There were a little Caspase-3-positive cells in the normal control group and sham operation group. Caspase-3 expression was increased at 6h, there was significant difference to compare the sham operation group (P<0.05), peaked at 3d and then declined, and after 11d there were still few posivite cells. There was a positive correlation between Caspase-3 expression and cell apoptosis in 11d after ICH (r =0.711, P<0.01). The Caspase-3-positive cells of hematoma aspiration group were fewer than those of natural recovery group at 3d and 11d, the difference was significant (P<0.05).3. There were no Cyt c-positive cells in the normal control group and a little positive cells sham operation group. Cyt c expression was increased at 6h, there was significant difference to compare the sham operation group (P<0.05), peaked at 3d and then declined. There was a positive correlation between Cyt cexpression and cell apoptosis in 11d after ICH (r =0.808, P<0.01). The Cyt c-positive cel ls of hematoma aspiration group were fewer than those of natural recovery group at 12 h, 24h ,7d and 11d, the difference was significant (P<0.05).Conclusion:1. Apotosis is a pattern of death after intracerebral hemorrhage, and Caspase-3, the important mediator of apoptosis, has an intimate relation with the brain tissue apoptosis induced by the hematoma.2.Apoptotic channel includes hondriosome and death receptor two channel after intracerebral hemorrhage, and the release of cytc from mitochondria to cytosol and Fas receptor are two critical step in apoptotic cell death after ICH.3. Hematoma aspiration can effectively alleviate apoptosis after ICH by preventing Cyt c and Fas release.
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