The Effect Of Stereotactic Hematoma Aspiration On The NSE Expression After Experimental Intracerebral Hemorrhage In Rat

Objective To observe the effect of stereotactic hematoma aspiration on the expression of neuron specific enolase(NSE)around hematoma cortex after intracerebral hemorrhage.Methods The model of intracerebral hemorrhage were established by using inracerebral stereotaxic injection of type IV collagenase method in male adult Wistar rats and were randomly divided into sham operation group, intracerebral hemorrhage group, and hemorrhage aspiration group. The neurological impairment in rats were evaluated by Bederson four grade. The brain water content was obtained by wet and dry weight method at 1d, 2d, 3d, 5d after intracerebral hemorrhage. The morphology of nerve cells in cortex were observed by the hematoxylin-eosin(HE)staining. The expression of NSE around hematoma cortex was detected respectively by the immunohistochemistry( IHC) and Western Blot.Results The expression of NSE around hematoma cortex in hematoma aspiration group and intracerebral hemorrhge group was significantly higher than that in sham operation group at 1d, 2d, 3d, 5d after intracerebral hemorrhage(P<0.05). The expression of NSE around hematoma cortex in hematoma aspiration group was slightly higher than that of intracerebral hemorrhage ground one day after intracerebral hemorrhage(P>0.05), but there was no significantly difference. Compared with intracerebra hemorrhage group, the expression of NSE around hematoma cortex in hemorrhage aspiration group was significantly decreased at 2d, 3d, 5d after intracerebral hemorrhage(P<0.05). Compared with hemorrhage aspiration group, the numbers of positive cells were significantly increased and the damage of the neurons around hematoma cortex was worsened than that in intracerebra hemorrhage group(P<0.05).Conclusion The expression of NSE around hematoma cortex after intracerebral hemorrhage was increased obviously after intracerebral hemorrhage. However, early hematoma aspiration could weaken the expression of NSE, reduce the number of neuronal degeneration cells, and improved neurobehavioral function significantly after intracerebral hemorrhage.