| Objective: investigate(1)Smad7 expression at different time in Renal Ischemia-Reperfusion Injury(IRI).(2)Influence to Smad7 expression after renal ischemia-reperfusion injury of rats given TGFβ1 antibody at different time.(3)Search new clinical index and method to forecast acute renal failure.Methods: 72 male SD rats were randomized to the following three groups: Sham operation group(group1,n=24, in this group,24 rats were divide into four subgroups according to the time of harvesting sample,each subgroup has 6 rats),Ischemia-reperfusion group (group2,n=24,in this group,24 rats were divide into four subgroups according to the time of reperfusion,that is 0h,4h,12h,24h after reperfusion,each subgroup has 6 rats),TGFβ1 antibody treatment group(group3,n=24,24 rats were divide into four subgroups as group2).Rats were anesthesized and undertook lapotomy. Sham operation group were treated without occlusion of left and right renal artery.In group2 and group3,right occlusion was performed as soon as occlusion of left renal artery for 45 minutes and then recanalization.TGFβ1 antibody were administered intravenously at a dose of 5mg/kg 2 hours before reperfusion in group3.The rats in other two groups were IV normal saline equivalently.When the experiment was finished,left nephrectomy was performed in every group,at the same time,blood samples were obtained for measurement of serum levels of creatine(Cr) and blood urea nitrogen(BUN). Renal tissue was used for histological examination and immuohistochemical analysis of Smad7.Results: (1)Renal function change: Compared with group1,the serumlevels of BUN in the subgroup were higher significantly in group2 and group3(P<0.01, P<0.01).Compared with group 2,the serum levels of BUN in the TGFβ1 antibody treatment subgroups of 0h reperfusion were not different statistically, and those in other subgroups were lower significantly in group3(P<0.01).Compared with group1,the serum levels of Cr in group2 and group3 were significantly higher than those in group1(P<0.01). Compared with group2,the serum levels of Cr in the TGFβ1 antibody treatment subgroup were lower significantly (P<0.01).(2)Renal cellular damages in group2 showed more severe than those in group1 and group3.(3)Expression of Smad7 in kidney of 0h reperfusion were not different statistically(P>0.05).Expression of Smad7 in kidney of group 2 were lower significantly in 4h,12h,24h reperfusion then group2 and group3. Expression of Smad7 in kidney of group3 were lower in 4h and 12h reperfusion then group1(P<0.01),expression of Smad7 in kidney of 0h and 24h reperfusion were not different statistically(P>0.05).Conclusion: 1.Expression of Smad7 in kidney of 0h reperfusion were not different statistically(P>0.05).Expression of Smad7 in kidney of group2 were lower significantly in 4h,12h,24h reperfusion then group2 and group3. Expression of Smad7 in kidney of group3 were lower in 4h and 12h reperfusion then group1(P<0.01),expression of Smad7 in kidney of 0h and 24h reperfusion were not different statistically(P>0.05). 2.Our results indicate that TGFβ1 antibody can attenuate renal ischemia-reperfusion injury.
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