BFGF Improves The Viability Of Subdermal Vascular Network Flap

Objective: As China becomes more and more developed, patients suffered from industry and agriculture accident increase. Many traumas incurred by accident including degloving injury, heat pressure injury bum injury etc. Combine with soft tissue defect,especially skin defect,and deep tissue exposure,which need to be covered or reconstructed by flap or free grafts on time. SVNF (Subdermal Vascular Network Flap) is a new member of flap family .The good texture of this flap makes it a best candidate for repairing skin defect on face or limb. where cosmetic need is emphsized. The other characteristics of SVNF include early pedicle division feasibility and safety. But the main limit of SVNF is its ratio of length to width,which can't be surpassed by 2.5:l,otherwise the distal part wil be necrosis. bFGF is a kind of glycoprotein which can enhance local endothelial cell proliferation vascular hyperpermeability and promote angiogenesis. We want to know if bFGF applied locally can improve the vascular reconstructiono of SVNF and the influence of applying dosage.. methods of bFGF on surrival rate of the flap. Methods Four white healthy pigs were used,weigh (20±2.5)kg , including male and female pigs.(1)the establishment of animal body:each pig had 6 SVNF On back,cut down the blood vessel from the abdomen milk line to form random pattern skin flaps. 16cm×4cm,the long axis of skin flap is vertical with the long axis.(2)Grouping :totally 24flaps were divided randomly into 2 groups. the bFGF groups and the control groups. the group of basic fibroblast growth factor: twelve random pattern subdermal vascular network flaps, the control group: twelve random pattern subdermal vascular network flaps,(3)enforce of the influential factor:bFGF(buy from the DONG DA biological engineering company),as the SVNF were formed,choose the points that 12cm, 14cm, 16cm from the stem of skin flap as injection points,to give subcutaneous injection. to handle as follows: the bFGF groups:heparinezed saline containing bFGF 0.1ml was injected in every point,0.6ml heparinezed saline containing bFGF was used. the control group: heparinezed saline without bFGF was injected in every point. 0.6ml heparinezed saline was used.After the injection of medication ,suture the skin flap in natural position,the distal 1/3 of the skin flap was packaged and pressured dressing. after the pigs clear-headed from the anaesthesia,every pig was feed in a cage,400 ten thousand penicilin was injected every day to prevent infection.(4)detection index: observation:on the 6^th postoperative day ,we demolish the package to observe the color ,texture and growth of pig hair of every group, to compute the survival rate of every group,histology observation: to take 1×1×1cm tissue from near middle and far 1/3 of every skin flap,to observe the change of histology under light microscope,calculate the density of vessel in the tissue.Result: The results suggest that the injection of bFGF locally can promote the survival rate by accelerating the blood circulation of the SVNF' bottom and edge. shorten the division time of SVNF and broaden the ratio of length and width.