Expression Of C-myb, NF-κB And Gene Rearrangement Of TCR γ In Cutaneous Lymphoma

Objective: To detect the expression level of c-myb and NF- κB p65 in cutaneous lymphoma and cutaneous inflammation as well as the TCR γ gene rearrangement in cutaneous lymphoma . And to discuss the expression of c-myb and NF-κB p65 in cutaneous lymphoma and cutaneous inflammation and the relationship between them, compared with the result of gene rearrangement of cutaneous lymphoma, to investigate the effect of the proteins on the diagnosis and differential diagnosis of cutaneous lymphoma. Then to explore the relationship between abnormal expression of c-myb and NF-κB p65 protein and the pathogenesis and development of cutaneous lymphoma.Methods: S-P Immunohistochemistry method was performed to examine the expression of c-myb and NF- κB p65 protein in 40 cases of cutaneous lymphoma and 15 cases of cutaneous inflammation. TCR γ gene rearrangement of lymphoma was performed in 22 cutaneous lymphoma to determine the diagnosis of cutaneous lymphoma.Results:1. c-myb protein: The positive expression rate was 77.5%(31/40) in cutaneous lymphoma and 0% in cutaneous inflammation. The positive expression rate between cutaneous lymphoma and cutaneous inflammation was different in statistics.(P<0.01). 2. NF-κB p65 protein: The positive expression rate was 90%(36/40) in cutaneous lymphoma and 73.3% in cutaneous inflammation. The positive expression rate between cutaneous lymphoma and cutaneous inflammation was not statistically significant.(P>0.05).However, the strong positive expression of NF-κB p65 rate between cutaneous lymphoma and cutaneous inflammation was different in statistics (P<0.01).3. The significant correlates was observed between the expression of c-myb and NF-κB p65(P<0.01).4. The expression of c-myb, NF-κB p65 protein was not correlated with the clinical staging of mycosis fungoides (P>0.05).5. The detection of gene rearrangement: TCR γ gene rearrangement was detected 12 in 22 of cutaneous lymphoma(54.5%).Conclusions:1. The abnormal expression of c-myb and NF-κB p65 protein are associated with the pathogenesis and development of cutaneous lymphoma.2. The expression of c-myb, NF-κB p65 protein was not correlated with the clinical staging of cutaneous lymphoma. The abnormal expression of c-myb and NF-κB p65 protein are occurred in early cutaneous lymphoma.3. The detection of c-myb, NF-κB p65 protein expression is helpful for the early diagnosis and differential diagnosis of cutaneous lymphoma. The positive expression of c-myb and (or) strong positive expression of NF-κB p65 highly hint cutaneous lymphoma.4. Negtive result of TCR- γ gene rearrangement can not exclude cutaneous T cell lymphoma.