| Marasmius androsaceus(L.ex Fr.)Fr. is called Gui-maozhen. It is a kind of fungus,it is belong to taxonomy, Agaricales, small white mushrooms Branch Marasmius. It grows on wood or bamboo sticks which are planted deep in the densely forested mountains where condition is cool and wet, acidic soil near the water.It is one of higher value Chinese medicines.Saponin of Marasmius androsaceus is as a medicial product of"An-Luotong". Marasmius androsaceus is artificial cultivated in liquid or solid state fermentation way in order to aquire industrial raw materials.Using bagasse,wheat bran or rice bran as a matrix for solid training.miao filaments with the matrix are being dried for the next steps. At present, the chemical composition has been found that it mainly including mannitol,cholesteryl acetate,amino acids,hydroxycinnamic acid, Ergosterol, Carbohydrates and protein components.Mycelium which has been solid trained can cure many diseases such as many kinds of neuralgia,Rheumatoid pain,neuritis an Rheumatoid Arthritis and so on.In these diseases it has made significant effect.Both ning pain medicine and network security pain are mainly made of Marasmius androsace -us have long been recognized the analgesic efficacy and lasting effect by the majority However, the active components for Hydroxycinnamic acid, Carbonate 30, Ergosterol,Diterpenes compounds,And Saponin has been reported has a feature of fast effect lasts a short time.This result is contradicted to the features of the clinical analgesic of Marasmius androsaceus. Although Maras mius androsaceus shows good efficacy for Sciatica, Trigeminal Neuralgia,Rheumatoid arthritis pain and so on.However, its active ingredient extraction,separation,purification and basic chemical composition of the active ingredient has not been resolved,The drug has long been impeding the development and use of raw materials.Meanwhile, Meanwhile, there are experiments show that Ethanol precipitation of Marasmius androsaceus may be a long time analgesic active ingredients.Marasmius androsaceus is a kind of medicin al fungi which has signifycant analgesic effect.The experiment is composed of three portions. First,more efforts are being expended in extracting and purifying polysaccharide of the medicinal fungi.Second, analysis the physicochemical propertyes, molecular weight, monosaccharide composition of all the portions which are and before,at the same time,do writhing analgesia pharmacological experiments in rats using all portions extracted and purified ,At last,analysis the Structure of one of the portions (MAEP3b).The experiment uses the method that extracting polysaccharide of Marasmius androsaceus from water,and then 80% ethanol precipitating to get MAEP. After that,MAEP is further isolated in three ways,①use (weakly basic anion exchange agent) DEAE sephadex A-50;②Hollow fiber filter (molecular weight 5000);③D101 macroporous resin.Next,the physico-chemical of all isolated portions are detected by spectrophotometry;molecular weight determination by differential refractive index detector and HPLC; monosaccharide composition analysis in the way of hydrochloric acid derivative method by GC.After that all the portions are used to do pharmacological in order to compare their inhibition activity by acetate leading writhing response in rats. At last analysis the structure of one of the portions (MAEP3b) by means of some parts of methylation and the others parts of acetylation method by GC—MS.The results show that the inhibitory role of MAEP is notable better than Marasmius androsaceus's. The total Carbohydrates,Protein and Uronic acid Content% of MAEP3b is 73%,44%,1.1%;molecular weight of MAEP3b distribution in the main 19180—187, one of the polysaccharide's molecular weight is 2891 which accounts for the largest proportion ,the ratio is 32.3%;Monosaccharide composition of MAEP3b (molar ratio) is:Man:Glu:Gal=3.4:36.2:1.0;The chain skeleton of MAEP3b is 1→2,1→3,1→4,1→6 linked Glu,1→4 linked Man,1→2 linked Gal;and there are branches at 4 position of 1→6 linked Glu or at 6 position of 1→4 linked Glu,and the 3 position of 1→4 linked Man;and the terminal is Glu.In addition, with the two methods of 1-methyl-3-phenyl-5 -Pyrazolone precolumn derivatization to Determinate the result of sugar component by Acetyl derivative used in gas chromatogramphy and HPLC. Contrast the two methods, the latter is simple, stable, small losses. higher sensitivity, better suited to trace determination of monosaccharide composition.
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