Marasmius Androsaceus Polysaccharide Extraction Process Optimization And Its Analgesic Effect

Marasmius androsaceus is a traditional medicine and edible fungus, with food, health care and medicinal value. This study used manipulative experiment to optimize the extraction technology of fungi polysaccharides in Marasmius androsaceus. In addition, the analgesic effect of Marasmius androsaceus fermentation product on nitroglycerin-induced migraine rats was detected. The main methods and resultsshowed that:1) Marasmius mycelium was chose as the object with an extraction process indicator of polysaccharide yields. The Box-Behnken response surface analysis was conducted to illustrate the effects of different ultrasonic treatment time, the ratio of material to liquid, sonication power, and their possible interactions on the extraction rates of polysaccharides. In addition, the treatment effects were simulated and described using a prediction model of multivariate quadratic regression equation. The results showed that the optimum extraction conditions were that ultrasonic extraction time, the ratio of material to liquid, and ultrasonic power were 26.43 min, 254.29 g/L, and 465.01 W, respectively, with 27.42% of marasmius polysaccharide yield.2) The alcohol extractionmethod of extracellular polysaccharide of Marasmius mycelium were optimized. First, we chose the optimal concentrate volume ratio of fermentation liquid, alcohol extraction time, and ethanol amount. To obtain the optimal separation and purification conditions of alcohol extraction method, the Box-Behnken response surface analysis, which goals were fermentation biomass and extracellular polysaccharides content, with three factors and three levels was conducted. The optimal conditions were: concentrate volume ratio of 0.29, the volume ratio of ethanol to fermentation liquid of 5.20, alcohol extraction time 12.34 h. Under these conditions, the quality of polysaccharides was up to 6 g/L.3) The groups of Marasmius mycelium, fermentation liquid, Anluotong, and Pisanjian were used to feed the nitroglycerin-induced migraine rats at different doses. Venous blood and brainstem samples were collected to detect rat venous blood and brainstem NOS and NO concentration. The results showed that Marasmius mycelium, fermentation liquid, and Anluotong can significantly suppressed NO and NOS concentration of venous blood and brainstem. However, the effect was slightly lower than Western medicine group, due to that the abirritation of Anluotong was slow in the clinical application. Pisanjian groups did not affect NO and NOS concentration of venous blood and brainstem.4) Marasmius mycelium, fermentation liquid, Anluotong, and Pisanjian groups were used to feed the nitroglycerin-induced migraine rats at different doses. Venous blood and brainstem samples were collected to detect rat venous blood and brainstem CGRP and β-EP concentration. The results showed that Aspirin, high concentration group, mid-concentration group, low concentration group, and Anluotong group can substantially suppressed CGRP and β-EP concentration of venous blood and brainstem, suggesting that Marasmius mycelium can cure migraine by regulating organism CGRP and β-EP concentration. However, fermentation liquid and Pisanjian groups did not affect CGRP and β-EP concentration of venous blood and brainstem.