Correlation Between The VEGF-C And MLVD Expression And Lymph Node Metastasis In Human Nasopharyngeal Carcinoma

Objective To detect the expression of VEGF-C and microlymphatic vessel density andprobe the relationship between lymphangiogenesis and lymph node micrometastasis innasopharyngeal carcinoma.MethodsThe expression of VEGF-C, was evaluated in 58 cases with nasopharyngeal carcinomaand 20 samples of nasopharyngitis tissues by immunohistochemical staining SPmethods. The microlymphatic vessel density(MLVD) in tumor was counted byimmunostaining with the specific antibody LYVE-1 and analyzed withclinicopathologic parameters.ResultsThe positive rate of VEGF-C was 84.48% and 15% respectively in nasopharyngealcarcinoma and nasopharyngitis tissues, there was significant difference betweenthem(P<0.05). The microlymphatic vessel density was 28.62+6. 21 and 10.53±2.99respectively in nasopharyngeal carcinoma and nasopharyngitis tissues, there wassignificant difference between them(P<0.05).The expression of VEGF-C and themicrolymphatic vessel density(MLVD) were significantly higher in nasopharyngealcarcinoma and in lymph node mesastasis group than in no-metastasis group. Theexpression of VEGF-C was positively correlated with MLVD(P<0.01),lymph nodemetastasis (p<0.01),clinical stage(p<0.05) , and it was negatively correlated with theprognosis of NPC patients,however not pathological grades(P>0.05).ConclusionsIn nasopharyngeal carcinoma ,the expression rate of VEGF-C was high. VEGF-Cexpression was positively correlated with MLVD and lymph node metastasis. VEGF-Cmainly promotes tumor lymphangiogenesis and induces to lymphatic metastasis. The detection of VEGF-C in nasopharyngeal carcinoma tissues will be significance forprediction of the treating and prognosis.