| [Objective]To observe the therapeutic effect of different doses of triclabendazole in rats infected with the juvenile worms of Pagumogonimus skrjabini. To investigate regularity of the dose-effect relationship. To probe into the best dosage of triclabendazole in rats infected with the juvenile worms of Pagumogonimus skrjabini. To explore ulteriorly the mechanism of triclabendazole to kill Pagumogonimus skrjabini.[Methods]60 rats were randomly divided into four groups: experimental group A,experimental group B,experimental group C and control group D, there were 15 rats in each group. The 60 rats were each injected through abdominal cavity with 15 metacercariae of Pagumogonimus skrjabini. Rats of experimental groups were treated with different doses of triclabendazole on the thirty-ninth day after infection respectly. Rats were dissected on the first,fifteenth,twenty-second day after finishing the treatment, each time there were 5 rats were dissected in each group. Recovered the worms, counting NO. of the worms recovered, calculating worm recovered rate and worm reduction rate, measuring the sizes of the worms recovered (length, width), weighing up wet weight of the worms recovered and compared with control group. Data were analyzed with SPSS11．5 statistic software. In addition, the structural changes of the worm tissues were observed under both light microscope and transmission electron microscope.[Results]Rats were dissected in the course of finishing the treatment, the results of the worms recovered showed that animal models had been made triumphantly. According to worm recovered rate,worm reduction rate: on the first day after finishing the treatment, there were no significant differences without exception between each other of rats in control group,low,middle and high-dose group (P>0．05) ; on the fifteenth,twenty-second day after finishing the treatment, there were significant differences between each other of rats in control group,low,middle and high-dose group (P<0．01) , but there were no significant differences between rats in low-dose group and control group on the fifteenth day after finishing the treatment (P>0．05) .Wet weight mensurated of the worms: on the first day after finishing the treatment, there were no significant differences without exception between each other of rats in control group,low,middle and high-dose group (P>0．05) ; on the fifteenth,twenty-second day after finishing the treatment, there were significant differences between each other of rats in control group,low,middle and high-dose group (P< 0．01) , but there were no significant differences between rats in middle and high-dose group on the fifteenth day after finishing the treatment (P>0．05) . The results measured on the sizes of the worms: on the first day after finishing the treatment, there were no significant differences without exception on the sizes of the worms(lengths width) between each other of rats in control group,low,middle and high-dose group respectly (P>0．05) ; on the fifteenth,twenty-second day after finishing the treatment, there were significant differences without exception on the sizes of the worms(length,width) between each other of rats in control group,low,middle and high-dose group (P<0．01) , but there were no significant differences on the sizes of the worms(length,width) between rats in low,middle and high-dose group on the fifteenth day after finishing the treatment (P>0．05) .Light microscope observation: low-dose group, body walls and intestinal walls were dropsical; middle-dose group, the external tegument dropped, smooth muscle exposed, parts of cortex cells degenerated,necrosised,dissolved, the cell bodies of gut epithelia dropped and decreased obviously; high-dose group, only muscle layer of body walls could be seen, cortex cells necrosised and dissolved, altitudinal swelling and looseness could be seen among parenchymal cells, gut epithelia dropped nearly outright, basal membranes of the intestinal walls could be seen oniy. Transmission electron microscope observation (dissected on the twenty-second day after finishing the treatment): triclabendazole had different extent breakage on ultrastructure of body walls and intestinal walls of the juvenile worms of Pagumogonimus skrjabini, the effect of low-dose group on the worms was slighter, the destructive effect of middle-dose group on the worms was stronger than that of low-dose group, but the destructive effect was not deadly, the destructive effect of high-dose group on the worms was deadly. The worms recovered of rats in control group showed no pathological changes.[Conclusions]Triclabendazole has remarkable therapeutic effect on rats infected with the juvenile worms of Pagumogonimus skrjabini, but the insecticidal course is tardigrade and the worms can be killed gradually. Following time pass by after the treatment, the therapeutic effect enhances gradually. Triclabendazole has remarkable dose-effect relationship on rats infected with the juvenile worms of Pagumogonimus skrjabini, when the doses of triclabendazole increase from 50 mg/kg. d to 200 mg/kg.d, the therapeutic effect enhances gradually, the best dose that triclabendazole treat rats infected with the juvenile worms of Pagumogonimus skrjabini is about 200 mg/kg. d. The mechanism of triclabendazole to kill Pagumogonimus skrjabini is that it can damage the tissue of both body walls and intestines, which result in handicap of both physiological function and biochemical metabolize of assimilation,absorb,exudation and excretion etc of parasite. The worms are killed or wounded finally.
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