The Effect Of Notogisenoside-Rg₁ On Variation Of BDNF MRNA On Focalcerebral Ischemia Reperfusion Injury In Rats

Objective:To study the effect of notoginsenoside-Rg₁ on expression of BDNF mRNA (brain-derived neurotrophic factor messenger ribonucleic acid, BDNF mRNA) in cerebrum cortex after MCAO/R (middle cerebral artery occlusion reperfusion, MCAO/R)injury in rat by real-time quantitative polymerase chain reaction. Methods:36 SD male rats, weighing 280-320 g, were randomly divided into MCAO/R model group,notogisenoside-Rgi therapy group(100 mg/kg) and the positive medicine control group(nimodipine 1mg/kg). The MCAO/R model rats were made by thread-occluded method. The four rats were randomly taken from each groups and were killed to be breaken out brain tissues as specimens after which were treated by medicine in 1,3,5 days. Total RNA was isolated from cerebrum cortex. Specific oligonucleotide primers of BDNF mRNA gene fragments were amplificated by RT-PCR. To construct recombinant plasmid and sequence. To dilute recombinant plasmid diploidly and a quantitative standard curve was completed. Taqman fluorogenic quantitative RT-PCR(FQ-PCR) was set up to Real-time RT-PCR was taken to detect the BDNF mRNA variety of cerebrum cortex . The data were analyzed by one-way Anova and (?) test. Results:Compare with the model group, notogisenoside-Rg₁ treated groups can obviously improve some nervous deficit symptoms in the cerebral ischemia and can increase BDNF mRNA amount that in the cerebrum cortex at different period after rat MCAO/R injury. Compare with the positive control group, notogisenoside-Rg₁ treated groups can obviously improve some nervous deficit symptoms in the cerebral ischemia and can increase BDNF mRNA amount that in the cerebrum cortex at different period after rat MCAO/R injury. Conclusion:Notoginsenoside-Rgi can promote to generating internal BDNF protein in brain by up-regulation the expression of BDNF mRNA amount in the cerebrum cortex. BDNF bind in TrkB that is its peculiar receptor, which can give rise to protective effects for ischemic neurons by generating corresponding domino offect molecules. Accordingly it can be as a therapy method in cerebral ischemia injury. Furthermore, the function of Notoginsenoside-Rg₁, are superior to that of the positive control medicine (nimodipine) both in going into operation time and effects during times.