Study On The Absorption, Fluorescence And Resonance Rayleigh Scattering Spectra Of The Interaction Between Halide Fluorescein Dyes With Some Drugs And Their Analytical Application

Resonance Rayleigh scattering (RRS), as a new analytical technique, is attracting more and more attention due to its high sensitivity and simplicity. In recent years, RRS has been applied to the determination of biological macromolecules such as nucleic acids, proteins, amylose. It also shows good application prospect in trace metal ions, organic compound and medicine analysis.The effect of the interaction between drugs and fluorescein halide dyes on the absorption, fluorescence and resonance Rayleigh scattering spectra, the optimum conditions of the reaction and the properties of analytical chemistry were investigated. The property of the scattering light was also studied by light polarization experiment. In addition, the reaction mechanism was discussed by AM1 quantum chemistry method.1. Study on the interaction between diphenhydramine and erythrosin by absorption, fluorescence and resonance Rayleigh scattering spectraIn pH 4.5 Britton-Robinson(BR) buffer solution, Erythrosin(ET) can react with Diphenhydramine(DP) to form an 1:1 ion-association complex, which not only results in the change of the absorption spectra, but also results in the great enhancement of resonance Rayleigh scattering(RRS) and the quenching of fluorescence. Furthermore, a new RRS spectrum will appear, and the maximum RRS wavelength were located at about 580nm. In this work, the spectral characteristics of the absorption, fluorescence and RRS, the optimum conditions of the reaction and the properties of an analytical chemistry were investigated. A sensitive, simple and new method for the determination of DP by using erythrosin as a probe has been developed. The detection limits for DP were 0.0020μg/ml for RRS method, 0.088μg/ml for absorption method and 0.094μg/ml for fluorophotometry. There was a linear relationship between the absorbance, RRS and fluorescence intensities and the drug concentration in the range of 0.0067～2.0,0.29～6.4 and 0.31～3.2μg/ml, respectively. The effects of the interaction of diphenhydramine and erythrosin on the absorption, fluorescence and Resonance Rayleigh Scattering Spectra were discussed. In light polarization experiment, the polarization of RRS at maximum wavelength was measured to be P = 0.9779, and it revealed that the RRS spectrum of DP-ET complex consists mostly of resonance scattering and few resonance fluorescence. In this study, enthalpy of formation and mean polarizability were calculated by AM1 quantum chemistry method. In addition, the reaction mechanism and the reasons for the enhancement of scattering spectra and the energy transfer between absorption, fluorescence and RRS were discussed.2. Study on the interaction between diphenhydramine and fluorescein dihalide dye by absorption, fluorescence and resonance Rayleigh scattering spectraThe interaction of diphenhydramine (DP) with three fluorescein dihalide dyes such as dichlorofluorescein(Cl₂-F), dibromofluorescein(Br₂-F), diiodofluorescein(I₂-F) and fluoreseein(F) was studied by absorption, fluorescence and resonance Rayleigh scattering(RRS) spectra. The results show that the diiodofluorescein and dibromofluorescein can react with diphenhydramine(DP) to form ion-association complexes, which not only results in the change of the absorption spectra, but also results in the great enhancement of RRS and the quenching of fluorescence. Furthermore, new RRS spectra will appear. But the above interactions can not carry out after mixing diphenhydramine with fluorescein and dichlorofluorescein. In this work, the spectral characteristics of the absorption, fluorescence and RRS, the optimum conditions of the reaction and the properties of an analytical chemistry were investigated. A sensitive, simple and new method for the determination of DP by using diiodofluorescein and dibromofluorescein as probes has been developed. DP-I2-F system has higher sensitivities than others, the detection limits for DP were 0.0035 Hg/ml for RRS method, 0.092μg/ml for spectrophotometry and 0.18μg/ml for fluorophotometry. There was a linear relationship between the absorbance, RRS and fluorescence intensities and the drug concentration in the range of 0.01～1.6,0.31～4.0 and 0.59～4.0μg/ml, respectively. The RRS method has the highest sensitivity in three methods and its sensitivity is 26 times and 51 times higher than spectrophotometry and fluorescence quenching method, respectively. Therefore, RRS method is more adapt to the determination of trace amounts of diphenhydramine. The effects of the interaction of diphenhydramine with diiodofluorescein and dibromofluorescein on the absorption, fluorescence and resonance Rayleigh scattering spectra were discussed. The property of the scattering light was studied by light polarization experiment, the enthalpy of formation, charge distribution and mean polarizability, were calculated by AM1 quantum chemistry method.In addition, the reaction mechanism and the reasons for the enhancement of scattering spectra and the energy transfer between absorption, fluorescence and RRS were discussed.3. Study on the interaction between Sildenafil and fluorescein halide dyes by absorption, fluorescence and resonance Rayleigh scattering spectraThe interaction of Sildenafil (SF) with five fluorescein dihalide dyes such as Ethyl eosin(EE), erythrosin (ET),. Eosin B(EB), Eosin Y (eY), diiodofluorescein(DI), dibromofluorescein (DB), Dichlorofluorescein(DC), Rose Bengal(RB) and Phloxine(PX) was studied by absorption, fluorescence and resonance Rayleigh scattering(RRS) spectra. The results show that the fluorescein halide dye can react with Sildenafil (SF) to form ion-association complexes, which not only results in the change of the absorption spectra, but also results in the great enhancement of RRS and the quenching of fluorescence. Furthermore, new RRS spectra will appear. EE-SF system has higher sensitivities than others, the detection limits for SF were 0.004μg/ml and 0.019μg/ml for RRS method and fluorophotometry, respectively. The spectrophotometry method of ET-SF system also has high sensitivitie, the detection limit for SF was 0.049μg/ml. The effects of the interaction of Sildenafil with fluorescein halide dyes on the absorption, fluorescence and resonance Rayleigh scattering spectra were discussed. The optimum conditions of the reaction and the properties of an analytical chemistry were investigated. In addition, the reaction mechanism and the reasons for the enhancement of scattering spectra and the energy transfer between absorption, fluorescence and RRS were discussed. A sensitive, simple and new method for the determination of SF by using EE as a probe has been developed.4. Study on the interaction between Raloxifene hydrochloride and fluorescein halide dye by absorption, fluorescence and resonance Rayleigh scattering spectraThe interaction of Raloxifene hydrochloride (RL) with five fluorescein dihalide dyes such as Ethyl eosin(EE), erythrosin (ET), Eosin Y (eY), diiodofluorescein(DI) and dibromofluorescein (DB) was studied by absorption, fluorescence and resonance Rayleigh scattering(RRS) spectra. The results show that the fluorescein halide dye can react with Raloxifene to form ion-association complexes, which not only results in the change of the absorption spectra, but also results in the great enhancement of RRS and the quenching of fluorescence. Furthermore, new RRS spectra will appear. EE-RL system has higher sensitivities than others, the detection limits for RL were 0.0036μg/ml, 0.021μg/ml and 0.050μg/ml for RRS method , fluorophotometry method and spectrophotometry method, respectively. The effects of the interaction of Raloxifene with fluorescein halide dyes on the absorption, fluorescence and resonance Rayleigh scattering spectra were discussed. The optimum conditions of the reaction and the properties of an analytical chemistry were investigated. In addition, the reactionmechanism and the reasons for the enhancement of scattering spectra and the energytransfer between absorption, fluorescence and RRS were discussed. A sensitive, simpleand new method for the determination of RL by using EE as a probe has beendeveloped.