Study Of The Expression Of MMP-9/TIMP-1 And The Relationship Between MMP-9/TIMP-1 And HBsAg, HCV Expression In Cancerous And Pericancer Tissues Of Primary Hepatocarcinoma

Objective To study the expression of MMP-9/TIMP-1 in cancerous and pericancer tissues ofprimary hepatocarcinoma and the relationship between their expression and liver cancerbiology behavior, HBsAg, HCV.Methods The patterns of MMP-9, TIMP-1 and HBsAg, HCV in 40 cases of PHC and theirsurrounding liver tissues were studied with paraffin sections using immunohistochemistrytechniques.Results The positive rate of MMP-9 protein was 72.5% and 75% in tumor and paratumorsamples, and the positive rate of TIMP-1 protein in carcinomar and the tissue close to tumorwas 57.5% and 62.5%, there were no differences between each other(P＞0.05). Both MMP-9and TIMP-1 were markedly increased than in normal liver tissues(P＜0.01). In the transferedgroup the expression of MMP-9 is much higher and the expression of TIMP-1 is markedlylower than in non-transfered in both cancerous and pericancer tissues of PHC (P＜0.05). HBsAgand HCV were detected both in PHC and pericarcinomatous tissues. The positive rate ofHBsAg in pericarcinomatous tissues (65%) was higher than that of in PHC tissues (27.5%);HCV expression in PHC (27.5%) and pericarcinomatous tissues (30%) had no differences. Theexpression of MMP-9 was guadually increasing from non-infection of HBV, HCV group topure HBV or HCV infected group to co-infection group in pericancer tissues of PHC. HBV,HCV virus infection were positively correlated with MMP-9(P＜0.05).Conclusions MMP-9 and TIMP-1 is over-expressed in both cancerous and pericancer tissuesof PHC, and they may have importmant value in determining whether there is metastasis ortransference; the expression of HBsAg and HCV in both cancerous and pericancer tissues mayhave different mechanism; HBV infection may increase the expression of MMP-9 in pericancertissues; HBV and HCV infection may lead transference by over-expressed MMP-9.