The Expression And Relationship Of Survivin And Caspase-3 In Non-small Cell Lung Cancer

Objective A novel inhibitor of apoptosis protein designated survivin has been found inmany tumor tissues,but not in normal tissues. Caspase-3 is a apoptosis protein.They areclosely associated with occurrence and development of malignant tumor.This study was conducted to investigate the expression and relationship of survivin andcaspase-3 in Non-small cell lung cancer.Material and Methods Use light microscope and immunohistochemical method todetect the expression of Survivin and caspase-3 in seventy-four paraffin-embeded NSCLsamples and to observe the relationship between the expression of the two factors.Result (1) Survivin was expressed in 48 of 74(64.9%) cases of NSCLC tissues. Theexpression of survivin gene was closely related to TNM stages, lymph node involvement,and cell differentiation, but not to histological classification, gender, and ages.(2) caspase-3 was expressed in 41of 74(55.4%) cases of NSCLC tissues. Theexpression of survivin gene was closely related to lymph node involvement, but not toTNM stages, histological classification, cell differentiation, gender, and ages.(3) The expression of survivin gene was closely related to the expression ofcaspase-3 in NSCLC tissues. A correlation coefficient test show an negtive correlationbetween the level of caspase-3 expression and the level of survivin expression in NSCLC.(r=-0.404, P＜0.01).Conclusion The over-expression surviving and the no-expression suggested the badprognosis in NSCLC. The over-expression survivin and de-activiaty of antioncogenecaspase-3 might play synergetic roles in the carcinogenesis of NSCLC.