Electrochemical Behavior Of Hemoglobin At Film Modified Glassy Carbon Electrode And Information Analysis

Hemoglobin is an important sort of biological macromolecular in human body and substantial basis of biological activity. Therefore, it's very important to research hemoglobin. Electrochemical methods are one of the beneficial tools exploring the biological proud mystery. It has widely application analysis, biological analysis and disease examination. As we know, it is difficult to respond on the ordinary electrode. With the development of the modified electrode, the electrochemistry research of protein is greatly promoted.In the process of biological electrochemical analysis, it's very important to acquire, select, dispose and conform the information. The thesis considers the electrochemical reduction system of Bovine Hemoglobin (Hb) on a glassy carbon electrode modified Lauric Acid, cholesterol, lecithin as the aim of the investigation. The reduction system employs the techniques of the cyclic voltammetry and AC impedance, furthermore, the thesis also focuses on the introduction of scientific methods. In order to do this research, we also use the computer techniques.The main CV work and the basic conclusions are outlined as follow:1. The character of Hb on the modified GC electrode was analyzed by the method of CV, which results show that the electrochemical deoxidization of Hb was an irreversible charge transfer system without strong adsorption .The peak current decreased with the constitute experiment. So in this system, there is the weak adsorption of electroactive species.2. According to the quantitative analysis of the data, it's difficult to predicate which is the dominant function between diffusion and adsorption. Maybe the diffusion is main factor.3. Add the fractal on the Michaelis-Menten model, we can get the relationship between the peak current ip and the concentration of Hb. So it can be concluded that the electrochemical deoxidization of Hb on modified electrode related to the fractal dynamics.In order to obtain some information, the experimental EIS data is treated with the help of EQU. Then, the information is analyzed and confirmed. The basic conclusions can be drawn upon:1. The process of this reaction probably relates to three variable parameters, and they refer to the direct current polar potential, the absorption of the Hb, and the statistic equal thickness of the membrane.2. In this reaction, the absorption and construction of the membrane play the important role in the dispersion effect, however, not the dielectric loss of the Hb.3. From this reaction, apart from the impedance of the electrode/solution interface, the impedance of the membrane caused by the non-Faraday.4. The electrode reaction system incompatible with the proliferation of conventional diffusion model. can be attributed to have a "barrier-proliferation" - the very scale. there are still reasons for the proliferation of non-conventional needs to be further explored. Testing has indicated that in view of cyclic voltammetry, Michaelis- Menten equation hypnosis to be the classic that the fractal model can be used to describe the peak current and concentration of the protein, thus initially assumed, the reason may be related to non-conventional proliferation by the acid in the cholesterol-lecithin-lauric acid modified glassy carbon electrode and the electro-catalytic reduction related to the fractal dynamics.