The Volt-ampere Characteristic Of The Interaction Of Amino Acids And Epinephrine And Theoretical Research

As one of the many bioactive macromolecules of the body, amino acid is building cells, the basic materials for repair organizations. Adrenaline is an important material of catecholamine neurotransmitters for the human and mammals. It controls the nerve system response to a series of biological and chemical processes. The research on molecular biological is to understand the interaction between biological systems and identify the specific role of the foundation. The study of the interaction between amino acid molecules and adrenaline are for the promotion of life sciences, pharmaceutical chemistry, physical chemistry. In this paper, we studied the influence of glycine, serine, glutamate, aspartic acid and phenylalanine on oxidation of epinephrine cyclic voltammetry (CV) curves with electrochem method, and focused on the electrochemical behaviour of epinephrine aroud isoelectric point of the aspartic acid .At the same time, we assisted in quantum chemistry methods to carry out the theoretical study of hydrogen bonding complexes formed between aspartic acid and adrenaline.Cyclic voltammetry was used to study the effect of glycine, serine, glutamate, aspartic acid and phenylalanine on the electrochemical properties of adrenaline in the hydrochloric acid buffer solution. The results indicated that five amino acid had stabilization on adrenaline, so that the ability of oxidation of adrenaline decreased, which altered the cyclic voltammetric properties of adrenaline. The ability of the five amino acids affect adrenaline differently for theirs structures different. Because ionic strength also has effect on the electro-oxidation behavior of epinephrine, we studied the five amino acid on the cyclic voltammetric properties of electron transfer of adrenaline in different ionic strength solutions. It showed that the five amino acid had different stabilization in different system to adrenalin, namely the different ionic strength of system had certain influence to the interaction bewteen amino acid and adrenaline. Simultaneously we had studied the aspartic acid emphatically around the isoelectric point the influence on the adrenalin oxidation behavior using the cyclic voltammetry and the alternating-current impedance. It indicated that aspartic acid presence varied along with pH value differred .Aspartic acid and its anion are the main object of study, we optimized their configurations. The hydrogen bonding complexes could be formed between adrenaline and them. The quantum studies were carried out in this thesis. The geometries of adrenaline and the stable complexes were respectively optimized to obtain structural parameters and binding energies. The calculation results could be used to explain some experimental phenomena better.