Compare The Adsorption Effect Of Endotoxin And Cytokine By Blood Perfusion With Several Sorbents

Objective: Campared the clearance effect of blood perfusion on endotoxemia rats by several sorbents, To make some helps for the development of a new clinical effective hemoperfusion sorbent to treat the endotoxemia.Methods: There were 48 SD rats assigned into 8 groups according to different sorbents:groupⅠ: synthesis macroporous adsorptive resin of single use hemoperfutor from LIVZON corporationgroupⅡ: AB-8 macroporous adsorptive resingroupⅢ: AB-8 macroporous adsorptive resin with diamine arms and coating with albumingroupⅣ: activated charcoal coating with albumingroupⅤ: ethyl cellulose coating with albumingroupⅥ: ethyl cellulose with glutaraldehyde arms and coating with albumingroupⅦ: ethyl cellulose with PME ligands and coating with albumingroupⅧ: ethyl cellulose with AMIKACINI ligands and coating with albuminEvery rat was injected 1ml/100g E. coli through jugular vein to set the model of endotoxemia, and 10ml blood was taked out after 1 hour. Then, the hemoperfusion was performed. When before hemoperfusion and after the first, second, third, fourth cycle, 2ml blood was picked out so as to detect the concentration of endotoxin, TNF-αand IL-6. Results:1 The clearance effect on endotoxin: Following the process of hemoperfusion, the concentration of ET of groupⅣ,Ⅴ,Ⅵ,ⅦandⅧwas lower and lower. There was significant difference between C4 and CO of each group, (p＜0.05). But in groupⅠ,ⅡandⅢ, the concentration after hemoperfusion was almost the same as the initial concentration, no significant difference between C4 and CO of each group (p＞0.05).And the result of contrasting cleanrance effect on endotoxin of sorbents was: groupⅦ＞groupⅣ＞groupⅧ＞groupⅥ＞groupⅤ＞groupⅢ＞groupⅡ＞groupⅠ. There was no significant difference between groupⅣand groupⅧ(p＞0.05), also no significant difference among groupⅠ, groupⅡand groupⅢ(p＞0.05), but significant difference was existed between each two others (p＜0.05).The groupⅦhad the best clearance effect on endotoxin. On the contary, the groupⅠhad the worst clearance effect.2 The clearance effect on TNF-α: Following the process of hemoperfusion, the concentration of TNF-αof groupⅠ,Ⅱ,Ⅲ,Ⅴ,Ⅵ,ⅦandⅧwas lower and lower, and descended obviously after hemoperfusion. There was significant difference between C4 and CO of each group, (p＜0.05). But in groupⅣ, the concentration of TNF-αafter hernoperfusion was almost the same as initial concentration. There was no significant difference between C4 and CO of each group(p＞0.05).The contrasting of cleanrance effect on TNF-αof each sorbents was: groupⅦ＞groupⅠ＞groupⅧ＞groupⅤ＞groupⅢ＞groupⅥ＞groupⅡ＞groupⅣ. There was no significant difference among groupⅤ,Ⅵ,ⅡandⅢ(p＞0.05); also no significant difference between groupⅠand groupⅧ, but significant difference was existed between each two others(p＜0.05).The groupⅦhad the best clearance effect on TNF-α. On the contary, the groupⅥhad the worst clearance effect.3 The clearance effect on IL-6: Following the process of hemoperfusion, the concentration of IL-6 of groupⅣ,Ⅴ,Ⅵ,ⅦandⅧdescended gradually. After hemoperfusion, the concentration descended obviously. There was significant difference between C4 and CO of each group(p＜0.05). But in groupⅠ,ⅡandⅢ, the concentration after hemoperfusion was almost the same as initial. There was no significant difference between C4 and CO of each group(p＞0.05).And the result of contrasting endotoxin cleanrance effect of each sorbents was: groupⅥ＞groupⅦ＞groupⅧ＞groupⅥ＞groupⅤ＞groupⅡ＞groupⅠ＞groupⅢ. There was no significant difference between groupⅤandⅥ(p＞0.05), also no significant difference between groupⅦandⅧ(p＞0.05), but significant difference was existed between each two others (p＜0.05).Conclusion: 1 Cleanrance effect on endotoxemia of sorbents was: ethyl cellulose with PME ligands and coating with albumin＞activated charcoal coating with albumin＞ethyl cellulose with AMIKACINI ligands and coating with albumin＞ethyl cellulose with glutaraldehyde arms and coating with albumin＞ethyl cellulose coating with albumin＞AB-8 macroporous adsorptive resin with diamine arms and coating with albumin＞AB-8 macroporous adsorptive resin＞synthesis macroporous adsorptive resin of single use hemopcrfutor from LIVZON corporation.The first three kinds of sorbent have nice clearance effect on endotoxemia. On the contrary, the finally three kinds of sorbent can almost not remove endotoxin.2 Cleanrance effect on TNF-αof each sorbents: ethyl cellulose with PME ligands and coating with albumin＞synthesis macroporous adsorptive resin of single use hemoperfutor from LIVZON corporation＞ethyl cellulose with AMIKACINI ligands and coating with albumin＞ethyl cellulose coating with albumin＞AB-8 macroporous adsorptive resin with diamine arms and coating with albumin＞ethyl cellulose with glutaraldehyde arms and coating with albumin＞AB-8 macroporous adsorptive resin＞activated charcoal coating with albumin.The first three kinds of sorbent have nice clearance effect on TNF-α. On the contrary, activated charcoal coating with albumin can almost not remove TNF-α.3 Cleanrance effect on IL-6 of each sorbents was: activated charcoal coating with albumin＞ethyl cellulose with PME ligands and coating with albumin＞ethyl cellulose with AMIKACINI ligands and coating with albumin＞ethyl cellulose coating with albumin＞ethyl cellulose with glutaraldehyde arms and coating with albumin＞AB-8 macroporous adsorptive resin＞synthesis macroporous adsorptive resin of single use hemoperfutor from LIVZON corporation＞AB-8 macroporous adsorptive resin with diamine arms and coating with albumin.The first three kinds of sorbent have nice clearance effect on IL-6. On the contrary, the finally three kinds of sorbent can almost not remove IL-6.In summary, activated charcoal coating with albumin, ethyl cellulose with PME ligands and coating with albumin and ethyl cellulose with AMIKACINI ligands and coating with albumin have nice clearance effect of blood perfusion on endotoxemia rats. On the contrary, AB-8 macroporous adsorptive resin, synthesis macroporous adsorptive resin of single use hemoperfutor from LIVZON corporation and AB-8 macroporous adsorptive resin with diamine arms and coating with albumin have bad clearance effect on endotoxemia.