| Diagnostic means of malignancy, sorts of tumor marker and investigationdirection of malignant early diagnosis have been summarized in this paper. And theauthor made out the investigation status quo for pterins through detection methodsand clinical applications. We established three new methods to mensurate theconcentration of pterins in both healthy people's urine and cancer's urine samples,which showed satisfactory results, and settled basement for discussing therelationship between pterins concentration and stomach cancer. In general, mainresearch results as follows:1. Simply introduced developing trend of malignancy, and explained the importanceof early diagnosis, then summed up some new techniques in pathology diagnosis. Wefurther illustrated the significance of using unique or multi-tumor marker to examinethe malignancy at early time. On the other hand, we showed the discussing directionof tumor diagnosis, which found to explore and look for new, differential, andhigh-sensitive tumor marker associated with more detection methods. At the sametime, there were some research of pterins in clinical study, especially for neopterinand biopterin more, this section summarized status quo of pterins in detectionmethods and applications.2. A high-performance liquid chromatography method was discussed in this section,we separated and determined neopterin, biopterin, pterin and isoxanthopterin at thesame time by using fluorescence detector. The HPLC conditions that we choosingwere: SHIMADZU chromatography column, Shim-pack: ODS (250×4.6 mm 5μm);mobile phase, methanol-water (7:93,Ⅴ:Ⅴ); flow rate, 1.0mL/min; column tempreture,room temperature; excitation wavelength, 350 nm; emission wavelength, 440 nm.Urine samples were analysed after filtrating by 0.45μm one-off filter. The recoveriesresults of healthy people's samples were neopterin (88.0%-106.2%), pterin(100.3%-102.6%), biopterin(93.2%-96.9%), isoxanthopterin(93.3%-102.4%), separately,whereas, recoveries of cancer's samples were neopterin (81.6%-103%), pterin(98.0% -99.8％), biopterin(94.8％-95.3％), isoxanthopterin(92.1％-103％), separately. Themethod could get satisfactory results when applied to analyse healthy and cancerpeople's urine samples.3. A synchronous fluorescence method was established to determine the xanthopterinin human urine. The measurement was carried out in a KH2PO4-NaOH buffer solution(pH=7.8) with△λ=70 nm for synchronous fluorescence scanning. Chemometricsmethods such as principal component regression (PCR), partial least squares (PLS),classical least squares (CLS) and radial basis function-artificial neural networks(RBF-ANN) were used to predict the concentrations of xanthopteim in artificialsamples and healthy people's urine. The results showed that PLS was the best, itsRSD was 4.29％. The methods, simple, rapid, andavoiding cumbersome pretreatment.The proposed method can be successfully applied to determine the content ofxanthopterin in human urine samples.4. Studied a synchronous fluorescence method to analyse the isoxanthopterin inhuman urine. The experiment was developed in a KH2PO4-NaOH buffer solution(pH=7.8) combined with△λ=65 nm for synchronous scanning, which can excludeinterferences of other substances in urine samples. The linear range for theconcentration of isoxanthopterin was found to be 0-1.4μg·mL-1. The linear regressionequation was Y=4105.3X+59.696, with linear correlation coefficient of 0.9997. Bothhealthy people's urine samples and patients' urine samples were measured in thispresent paper. For healthy people, the recoveries were in the range of 102.9％-108.4％, the relative standard derivations (RSD) were 0.37％-1.25％, while for thepatients' recoveries were in the range-of 85.6％-95.6％, and the RSD were 1.01％-2.25％. The method successfully applied to determine the concentration ofisoxanthopterin, and we adopted Significance Tests to found the notable differencesbetween healthy and stomach cancer urine samples in which we determined.
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