Study On Analytical Methods Of Peterins In Human Urine

Diagnostic means of malignancy, sorts of tumor marker have been summarized in this paper. And the author made out the investigation status quo for pterins through detection methods and clinical applications. We established three new methods to mensurate the concentration of pterins in both healthy people's urine and cancer's urine samples, which showed satisfactory results, and settled basement for discussing the relationship between pterins concentration and stomach cancer. In general, main research results as follows:1. Simply introduced developing trend and harmof malignancy, and explained the importance of early diagnosis, then summed up some techniques in pathology diagnosis. We further illustrated the significance of using tumor marker to examine the malignancy at early time. On the other hand, we showed the discussing direction of tumor diagnosis, which found to explore and look for new, differential, and high-sensitive tumor marker associated with more detection methods. At the same time, there were some research of pterins in clinical study, especially for neopterin and biopterin more, this section summarized status quo of pterins in detection methods and applications.2. A synchronous fluorescence method was studied to determine the isoxanthopterin human urine. The measurement was carried out in a KH₂PO₄-NaOH buffer solution(pH8.0)with△λ=65 nm for synchronous scanning, which can exclude interferences of other substances in urine samples. The linear range for the concentration of xanthopterin was found to be 0.011-0.56μg/mL.The linear regression equation was Y=6637.4X-10.988,with linear correlation coefficient of 1.The linear range for the concentration of isoxanthopterin was found to be 0.022-0.43μg/mL.The linear regression equation was Y=10763X-1.3713,with linear correlation coefficient of l.Both healthy people's urine samples and patients'urine samples were measured.For xanthopterin of healthy people,the recoveries were in the range of 89. 8%-90. 3%, the relative standard derivations(RSD)werel. 76%-2.15%, while for the patients'recoveries were in the range of 90. 0%-90. 8%,and the RSD were 1.50%-3.19%.For isoxanthopterin of healthy people,the recoveries were in the range of 84.3%-95.5%,the relative standard derivations(RSD)werel.44%-3.75%,while for the patients'recoveries were in the range of 92.3%-99.7%,and the RSD were 0.70%-1.54%.The proposed method was successfully applied to the determination of xanthopterin and isoxanthopterin in human urine samples.3.The high-performance liquid chromatography with fluorescence detection method was developed for determination of pterin-6- carboxylicacid in human urine. The conditions of HPLC were that: the mobile phase was acetonitrile-water (V: V=5:95), the flow rate was 0.8mL/min, the excitation and emission wavelengths were 360nm and 440nm. The linear range was 0.007μg/mL-2.4ug/mL.The linear regression equation was y = 394.55x + 1.24(r=0.9999), with the detection limit of 0.002μg/mL.For pterin-6- carboxylic acid in human urine, the recoveries were in the range of 101.3%-104.6%and the relative standard derivation(RSD) were under 7.1.The proposed method was successfully applied to the determination of pterin-6-carboxylicacid in human urine.4.Established a new High-Performance Liquid Chromatography - Ultraviolet detection of pterin-6-carboxylic acid in human urine. After being filtrated, urine samples were analyzed directly, and were separated on a Lichrospher C18 column by using methanol-water (70:30 ,V/V) as mobile phase, at the speed of 0.4 mL/min and the detected wavelength was 355 nm. The proposed method has successfully eliminated the disturbances in urine, and applied to determine the content of pterin-6-carboxylic acid in both healthy human urine samples and the cancers'. The intensity of pterin-6-carboxylic acid was proportional to the concentration of pterin-6-carboxylic acid over the range of 0.19-4.8μg/mL, with the linear correlation coefficient of 0.9999. The average recoveries were between 94.6% and 100.2%, and the relative standard detection was less than 5.07%.5.The determination influence of potentials has studied systematically ,explore chromatographic separation conditions of pterin - 6- carboxylic acid in human urine ,has established a new method-the ion chromatograph for the determination of pterin-6- carboxylic acid in human urine. The linear range for the concentration of pterin-6-carboxylic acid was 1.0μg/L-100μg/L.The linear regression equation was A=0.0318C+0.0277(r=0.9999), with the detection limit of 0.5μg/L.Applied to actual urine analysis with satisfactory.