Study On The TNF-α And Cholecyst's Macrophase When Ductus Choledochus Arose The Acute Choiecystitis And Relieve The Ligation

Objective:Ductus choledochus can arose the acute choiecystitis. Therefore, how know the mechanism of The common bile duct arose the acute choiecystitis. Explore the prevention and light the suffers pain ,avoid the suffers meet with operation is the important intention in this expe- rimentation This experiment adhibit rabbit creat the common bile duct animal model. Observe the ligation different time and after releasing from ligation.The change with the rabbet's temperature.White cell cholecyst's macrophage. TNF-αand TNF-αin blood serum .Discuss the ligation of common bile duct how affect the cholecystitis. Deep its understand of the pathogenesis.Methods: 72 healthy rabbits weighing 2.0-2.5Kg were randomly divided into 9 groups equally; the common comparison group(N)(n=8), the group of bile duct ligation one day(D1) (n=8), the group of bile duct ligation three days(D3) (n=8), the group of bile duct ligation five days(D5) (n=8), the group of bile duct ligation seven days(D7) (n=8), treating group: relieve ligation after bile duct ligation one day(R1) (n=8), relieve ligation after bile duct ligation three days(R3) (n=8), relieve ligation after bile duct ligation five days(R5) (n=8), relieve ligation after bile duct ligation seven days(R7) (n=8). All of the rabbits can fast before the operation. After creating model lay them into the cage, examine the rabbits'temperature different time. The all team of the rabbits get vein blood before executing, check the white blood cell. Radiation immunity measure the blood serum TNF-αcull the cholecyst after executing. Make them prepare olefin slice. Adhibit immunity chemistry measure the cholecyst's macrophase. Adope a little of cholecyst measure the TNF-αin it. All of the dates adhibit SPSS for window 13.0 software data deal with.The result use equal standard errand show. P<0.05 consider it is statistics significance.Result : (1)White blood cell: Common bile duct ligation team along with time .The white blood cell gradually ho- ist .After release the obstruction then fall . They don't release more hoister than the common comparison team. the release ligation team is different than the ligation team(p<0.05),the white blood cell of the release ligation one day team faller than the ligation one day team,is different(p<0.05). the release ligation three days team faller than the ligation three days team,is different(p<0.05). the release ligation five days team faller than the ligation five days team,is different(p<0.05). the release ligation seven days team faller than the ligation senen days team,is different(p<0.05).(2)Temperature:common bile duct ligation team along with time .The temperature a gradually hoist .After release the obstruction then fall . The don't release more hoister than the common comparison team.the ligation teams are different than the common comparison team(p<0.05),between of common bile duct ligation teams are different(p<0.05),the release ligation team is different than the ligation team(p<0.05),the temperature of the release ligation one day team faller than the ligation one day team,is different(p<0.05). the release ligation three days team faller than the ligation three days team,is different(p<0.05). the release ligation five days team faller than the ligation five days team,is different(p<0.05). the release ligation seven days team faller than the ligation senen days team,is different (p <0.05).(3)TNF-α:common bile duct ligation team along with time . serum and cholecyst's TNF-αgradually hoist .After release the obstruction then fall . The don't release more hoister than the common comparison team.the ligation teams are different than the common comparison team(p<0.05),between of common bile duct ligation teams are different(p<0.05),the release ligation team is different than the ligation team(p<0.05), serum and cholecyst's TNF-αof the release ligation one day team faller than the ligation one day team,is different(p<0.05). the release ligation three days team faller than the ligation three days team,is different(p<0.05). the release ligation five days team faller than the ligation five days team,is different(p<0.05). the release ligation seven days team faller than the ligation senen days team,is different(p<0.05).(4)Macrophase: common bile duct ligation team along with time . cholecyst's macophase gradually hoist .After release the obstruction then fall . The don't release more hoister than the common comparison team.the ligation teams are different than the common comparison team(p<0.05),between of common bile duct ligation teams are different(p<0.05),the release ligation team is different than the ligation team(p<0.05), cholecyst's macrophase of the release ligation one day team faller than the ligation one day team,is different(p<0.05). the release ligation three days team faller than the ligation three days team,is different(p<0.05). the release ligation five days team faller than the ligation five days team,is different(p<0.05). the release ligation seven days team faller than the ligation senen days team,is different(p<0.05)Conclusions : first: common bile duct ligation cause the rabbit acute cholecystitis . can hoist the white blood cell and temperate.increase serum and cholecyst's TNF-α,and cho- lecyst's macophase .second : After relieve the ligation can cure this kind acute cholecystitis. decrease he white blood cell and temperate . decrease serum and cholecyst's TNF-α,and cholecyst's macophase .