| Objective: To investigate the effection of the transection of the cervical sympathetic trunk(TCST) to rats'ulcerative colitis(UC). The pathogenesis of UC remains unclear up to now. It is generally accepted that UC is the result of interaction of multiple factors, Recent reports reveal that UC cases have increased substantially. At present, there is no radical cure for UC.Corticoid,aminosalicylic acid and immuno-inhibitors preparations are usually used to control and suppress the inflammation.Stellate ganglion block is a more commonly used clinical anesthesia technology, in addition to widely used for pain treatment of the disease, are increasingly widely used in other neurological, endocrine and immune obstacle in the treatment of diseases such as pain, and has achieved good results.The transection of the cervical sympathetic trunk in rat is a kind of ideal animal model to long-term SGB. This study is to investigate the effect of TCST on the UC.Methods: Experiments were performed in adult male Wistar rats weighing 245±5g. Seventy-two rats were randomly divided into three groups. Normal control group, Group A, model control group, Group B, TCST group, Group C, and each group had only 24. Adaptability kept in the laboratory for one week, before the experiment began with the production:First prepare UC model: Before experiment animals in each group were fasted for 48hours with free access to drinking water. Under sodium pentobarbital (30 mg/kg intraperitoneally) anaesthesia, and polyethylene cannula was inserted into the lumen of the colon via the anus, the catheter was introduced anally to a distance of 8cm. In the colitis group, 1ml of acetic acid was slowly infused into the distal colon and the rat was maintained in a head-down position for 30s to limit expulsion of the solution. Control rats were treated identically but instead of 4% acetic acid they received 1ml 0.9 % saline infusion. Each rat received a 3-5ml saline (0.9 %) flush followed by manual palpation of the abdomen twice.After model 24h, the rats were neck shed, conventional disinfection,median incision in cervical part, blunt separation subcutaneous tissue, fascia and muscle, at the inner margin of the sternocleidomastoid and latero-trachea carotid artery, the greyish yellow vagus nerve and much smaller white coarse sympathetic can be seen; in the ventral surface of the carotid artery bifurcation shows a slight expansion of the superior cervical ganglion. Group A and group B were exposed to the right cervical sympathetic trunk and superior cervical ganglion, wihtout mutilating the cervical sympathetic trunk then sutured the incision. Group C severed cervical sympathetic trunk 3mm in the patch below the superior cervical ganglion, and ligated the end, sutured the incision. Severed side were immediately with varying degrees of ptosis, rima oculi narrowing, contraction of pupil, auricle and forelimb high fever, but no significant change in the contralateral. And this proved TCST model successful.Since the day of the model, observe the weight and general condition of rats, and in the after three days, seven days and fourteen days only 8 in each group were selected, after 12-hour fasting blood,cardiac puncture to obtain the blood,then separate the serum to detect biochemical indicators: Determination of serum IL-6 with balance method, determination of NO with nitrate reductase method and iNOS activity with L-arginine (Arg) method, xanthine oxidase method for the activity of SOD; take all colon visual observation and scoring the gross morphological damage,the tissues were collected to the naked eye impressive ulceronecrotic about 500 mg, 10% of the same tissue were made using the above method of IL-6, NO,iNOS and SOD activity; keep the most obvious affection of the tissue 2cm x 2cm piece of 2-3, 10% formalin fix, make pathological section.Results: Compared with group A, the contration of IL-6,NO and iNOS activity in serum and in the gut tissue increased significantly in Group B, C(P <0.05), however, in group C increased significantly lower than that of group B (P <0.01); Compared with group A, the activity of SOD in serum and in the gut tissue decreased significantly in Group B, C(P<0.05), however, the extent of reduction in group C was significantly lower than that in group B (P <0.01).General state of Animal Model control group of rats with virtually no feces discharged from the previous 3 days, we can see that depressed,piloe-drection, extrado, crouching decub and few moving, anorexia; after 3 days, there was barely unshapen loose stools, the frequency of stools increased, accompanied by bloody pus and mucosa. The texture of anal mucosa turned embrittlement, easy to hemorrhage when touch it; 14 days there stool forming about 50%. In TCST group the general symptom relieve than the control group, improve the spirit and occasionally loose stool from the previous 3 days; 7 days less stool forming (about 30%); 14 days the group of TCST stool form as much as 80%. Normal control rats lively movability, fur bright, normal diet, with no death.The macroscopic observation and pathological changes In rat intestinal mucosa 3days in groupB colon general observation, segmental distribution of aphtha, mucous hyperemia and edema, thickening of bowel wall and enteric cavity stenosis. Endoscopic varying degrees of focal erosion, glandular mucosa damage, a large number of neutrophils in the lamina propria mucosae, slight eosinophilic infiltration, and some appeared crypt abscess. General observation in group C the ulcer reduction, mucosal congestion and edema lighter than in the group B. Endoscopic mucosal surface to cause in punctiform erosion and ulceration of lesions reduced, neutrophil decrease. 7 days, 14 days shows that ulcer's adjacent in rats of the two groups emerged epithelial repair, glandular hyperplasy, focal erosion and the infiltration of inflammatory cells gradually reduce, but the degree of change in TCST group is stronger than in model group. General observation of the colon tissue in model control group is that the texture of mucosal fold clear, without erosion and ulceration. Observation by light microscope see that the epithelium mucous membrane of colon integrity, continuous, the spread of gland regulation;structure distinct, secrete function active, mucous membrane, lamina propria blood vessel fiber mesenchymal normal, muscular layer with no abnormalitiesConclusion : TCST can reduce the content of NO, IL-6 and iNOS activity and enhance its activity of UC rats, and thus reducing the damage to the colonic mucosa, and promote the resumption of UC.
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