Studies On Protective Effect And Its Mechanisms Of Se-enriched Lactobacillus On Animals With Subacute Hepatic Injury And Hepatic Fibrosis

In current present paper, the subacute hepatic injury ICR mice and hepatic fibrosis SD rats, which were protected or turnovered by Se-enriched lactobacillus, were induced by abdominal cavity and hypodermic injection of chemical pollutant CCl₄, respectively. From immunology, cytology, physiology and toxicology angles, effects of Se-enriched lactobacillus on some immunologic cell activity as well as immune mediator levels and status of liver lipid proxidation were synthetically investigated by means of modern biology techniques to discuss protection mechanism protective effect, turnover and its mechanisms of Se-enriched lactobacillus on animals CCl₄-induced hepatic injury and hepatic fibrosis. The results were summarized as follows:1 Turnover effect and its immunoregulative mechanisms of Se-enriched lactobacillus on subacute hepatic injury miceThirty healthy ICR mice with half of female and male were chosen and randomly divided into a control group (group C), a CCl₄-injected group (group CCl₄) and a CCl₄ plus Se-enriched lactobacillus group (group CCl₄-Se). Subacute hepatic injury mice were given abdominal cavity injection of CCl₄ for over four weeks. After injection, the mice in group CCl₄-Se were given the diets with Se-enriched lactobacillus for over five weeks. The results showed that pathological specimen from group CCl₄-Se showed fibrogenesis was much less severe than that from group CCl₄ using HE staining. Plasma AST, ALT andγ-GT activities of group CCl₄ were higher than that of group C, but group CCl₄-Se was lower or significantly lower than group CCl₄. GSH-Px activity in liver homogenate in group CCl₄ was still lower than that in group C, but the enzyme activity in group CCl₄-Se was significantly superior to that in groups CCl₄ and C. Plasma TNF-αand IL-2 levels rose in group CCl₄ compared to group C, and dropped in group CCl₄-Se compared to group CCl₄. In group CCl₄, the level of spleen CD4⁺, CD8⁺ T-lymphocytes(%) and CD4⁺/CD8⁺ increased or notably increased compared to group C, but the values of group CCl₄-Se were close to those of group C. The spleen T lymphocyte stimulating index in group CCl₄ was slightly low to that in group C, however, group CCl₄-Se was significantly superior to groups CCl₄ and C. It was suggested that Se-enriched lactobacillus could effectively alleviate the subacute hepatic injury induced by CCl₄ by ameliorating and enhancing immunologic function activity in the body.2 Intervention effect and its immunoregulative mechanisms of Se-enriched lactobacillus on hepatic fibrosis in ratsThirty-six healthy SD rats with half of female and male were chosen and randomly divided into a control group (group C), a CCl₄-injected group (group CCl₄) and a CCl₄ plus Se-enriched lactobacillus group (group CCl₄-Se). Groups CCl₄ and CCl₄-Se were given hypodermic injection of CCl₄ to induce hepatic fibrosis on every other day for over eight weeks. During the entire experimental period, the animals in group CCl₄-Se are given the diets with Se-enriched lactobacillus. The results showed that during the whole experimental period, the hepatic index, which was the highest in group CCl₄, was lower or significantly lower in group CCl₄-Se than in group CCl₄ but notably higher than in group C. Plasma AST and ALT activities of group CCl₄ were higher or significantly higher than that of group C, however, a better decrease was observed in group CCl₄-Se. TP and AL levels in group CCl₄-Se were superior to group CCl₄. Compared to group C, GSH-Px and SOD activities in liver homogenate in group CCl₄ decreased significantly, but there was a higher or significantly higher value in group CCl₄-Se than in the group CCl₄ and the level was close to that of group C. The contents of MDA and Hyp in group CCl₄ rose compared to group C, but group CCl₄-Se was low to group CCl₄. Plasma TNF-αand IL-6 levels were higher and lower in group CCl₄ than in group C, and dropped and rose in group CCl₄-Se compared to group CCl₄, T-lymphocytes(%) of group CCl₄ significantly decreased. T-lymphocytes(%) of group CCl₄-Se, which were lower than those of group C, showed an apparent increase compared to those of group CCl₄. B-lymphocytes(%) in three groups was opposite to T-lymphocytes(%). RBC-CR1 rosette rates(%) and RBC-IC rosette rates(%) of group CCl₄ were lower or significantly lower than those of group C. However, group CCl₄-Se were markedly lower than in group CCl₄ and close to group C. During the entire experimental period, compared to group CCl₄, the NK cell activity and spleen T lymphocyte stimulating index in group CCl₄-Se significantly increased, but were still lower than that in group C. There were obviously fluctuations of spleen CD4⁺ and CD8⁺ T lymphocyte (%) as well as CD4⁺/CD8⁺ in group CCl₄, and Se-enriched lactobacillus alleviated the changes. It was suggested that Se-enriched lactobacillus could effectively intervene the production and development of hepatic fibrosis by protecting antioxidase activity in liver tissue, and ameliorating and enhancing immunologic function activity in the body.3 Turnover effect and its immunoregulative mechanisms of hepatic fibrosis in rats intervened by Se-enriched lactobacillusThirty-eight healthy SD rats with half of female and male were chosen and randomly divided into a control group (group C), a CCl₄-injected natural turnover group (group CCl₄) and a CCl₄-injected turnover group intervened by Se-enriched lactobacillus (group CCl₄-Se). Rats in groups CCl₄ and CCl₄Se were given hypodermic injection of CCl₄ to induce hepatic fibrosis on every other day for over eight weeks. Then animals in group CCl₄-Se are given the diets with Se-enriched lactobacillus for 6 wk to investigate the effect of natural turnover and Se-enriched lactobacillus intervened-tumover in hepatic fibrosis rats. The results showed that during the entire turnover period, the hepatic index of groups CCl₄ and CCl₄-Se gradually dropped but a stronger decrease existed in group CCl₄-Se compared to that in group C. Plasma AST and ALT activities in groups CCl₄ and CCl₄-Se decreased with increased of turnover time, and a bigger decrease was seen in group CCl₄-Se than in group CCl₄. GSH-Px activity in liver homogenate in group CCl₄ decreased or significantly decreased compared to group C though an enhanced value appeared. However, there was a lower value in group CCl₄-Se than in the group C in the 2nd week, the value was close to that of group C in the 6th week. During the entire turnover period, SOD activity in liver homogenate, which was the lowest in group CCl₄, showed a higher level in group CCl₄-Se. Liver homogenate MDA content was higher in group CCl₄ than in group C, and lower in group CCl₄-Se than in group CCl₄. Plasma TNF-αlevel was still higher in group CCl₄ than in group C though a decreased value existed, and plasma TNF-αin group CCl₄-Se changed between other both groups. Plasma IL-6 level was higher in group CCl₄, the alteration in group CCl₄-Se was close to that in group C. NK cell activities in groups CCl₄ and CCl₄-Se showed a lower levels at the 2nd week of turnover beginning, and increased with proceeding of experiment, and NK cell activity in group CCl₄-Se was always high to that in group CCl₄. The spleen T lymphocyte stimulating index in group CCl₄ was low to that in group C during the entire turnover period. However, group CCl₄-Se was superior to group C in the 2nd week and the alteration between other both groups was observed in the 6th week. During the entire turnover period, there were obviously fluctuations of spleen CD4⁺ and CD8⁺ T lymphocyte (%) as well as CD4⁺/CD8⁺ in groups CCl₄, and Se-enriched lactobacillus could effectively regulate the changes. It was suggested that compared to natural turnover, Se-enriched lactobacillus could more effectively accelerate the turnover of hepatic fibrosis by enhancing immunologic function activity in the body.