To Study The Effect Of Oxaliplatin On Human Cervical Cancer Hela Cells In Vitro

Objective To study The effect of oxaliplatin on growth inhibition and Caspase-3 activity on human cervical cancer Hela cells. Methods Human cervical carcinoma HeLa cells were treated with Oxaliplatin. Cell growth rate was determined with MTT assay, cell morphologic changes were examined under electron microscopy. Flow cytometry and TUNEL Was used to examine cell apoptosis rate. Activities of Caspase-3 were determined by chromometry , and its inhibition test by AC-DEVD-CHO. Results Oxaliplatin (4～64μmol/L) induced cytotoxicity and apoptosis to human cervical carcinoma HeLa cells in vitro is time-dependent and dose-dependent. The typical apoptotic morphological features appeared in cells treated with oxaliplatin for 48 hours; Caspase-3 activity was up-regulated in Oxaliplatin-induced apoptotic cells. The oxaliplatin induced HeLa cell apoptosis was efficiently inhibited by AC-DEVD-CHO. Conclusions Oxaliplatin can inhibit HeLa cell growth of cervical carcinoma and induce apoptosis in vitro. Caspase3 activation may be involved in oxaliplation-induced apoptosis of HeLa cells.