| Gastric cancer is the common tumor. Its occurrence, development isnot only related with the abnormal differentiation, tumor cell proliferation,but also associated with reduction of apoptosis. Therefore, finding the drugwhich could induce apoptosis of tumor cells and has small side effects is ofgreat significance. The purpose of this study is to explore the effect ofcurcumin and oxaliplatin alone or in combination on the growth inhibitionand apoptosis-inducing situation of human gastric cancer cells BGC-823, toobserve the changes of the expression of Fas protein and the activation ofCaspase-3in human gastric cancer cells BGC-823after treatment bycurcumin and oxaliplatin alone or in combination.Firstly, human gastric cancer cells BGC-823were treated by differentconcentrations of curcumin and oxaliplatin alone or in combination. After24hours, CCK-8assay was performed to detect the proliferation. CCK-8assay discovered that different concentrations of curcumin and differentconcentrations of oxaliplatin could inhibit the proliferation of humangastric cancer cells BGC-823. With the increase of drugs’ concentration,the inhibition rate gradually increased. The inhibition rate of the combinedgroups were significantly higher than the curcumin or oxaliplatin treatedgroups.Secondly, Human gastric cancer cells BGC-823were treated bycurcumin (20μmol·L-1) and oxaliplatin (10μmol·L-1) alone or incombination. After24hours, flow cytometry (Annexin V-FITC/PI staining)was performed to detect the apoptotic rate. Human gastric cancer cellsBGC-823were treated by curcumin (20μmol·L-1) and oxaliplatin (10μmol·L-1) alone or in combination. After24and48hours, DNA agarosegel electrophoresis was performed to observe the situation of apoptosis.Flow cytometry discovered that curcumin and oxaliplatin could induceapoptosis of human gastric cancer cells BGC-823. The apoptosis rate of thecombined group was significantly higher than the curcumin or oxaliplatintreated groups. DNA agarose gel electrophoresis displayed that thenegative control group, curcumin group, oxaliplatin group and thecombined group treating human gastric cancer cells BGC-823after24hours showed no obvious DNA ladder. However, after48hours, thecombined group appeared DNA ladder, and the drug alone group alsoappeared DNA Ladder but not obvious.Finally, in order to explore the molecular mechanism of curcumin andoxaliplatin induced apoptosis of gastric cancer cells, the expression of Fasand the activation of Caspase-3were detected. Immunocytochemistrydiscovered that curcumin and oxaliplatin could increase the expression ofFas in human gastric cancer cells BGC-823. The expression of Fas in thecurcumin or oxaliplatin treated groups and the combined group weresignificantly higher than the negative control group. The expression of Fasin the combined group was significantly higher than the curcumin oroxaliplatin treated groups. Flow cytometry discovered that curcumin andoxaliplatin could increase the expression of Fas in human gastric cancercells BGC-823. The expression of Fas in the curcumin or oxaliplatintreated groups and the combined group were significantly higher than thenegative control group. The expression of Fas in combined group wassignificantly higher than the curcumin or oxaliplatin treated groups.Spectrophotometry discovered that curcumin and oxaliplatin could increasethe activation of Caspase-3in human gastric cancer cells BGC-823. Theactivation of Caspase-3in the curcumin or oxaliplatin treated groups andthe combined group were significantly higher than the negative control group. The activation of Caspase-3in the combined group was significantlyhigher than the curcumin or oxaliplatin treated groups.As shown above, curcumin and oxaliplatin could significantly inhibitthe proliferation of human gastric cancer cells BGC-823in aconcentration-dependent manner and induce apoptosis. The mechanism ofthese effects possibly related with the two drugs could up-regulate theexpression of Fas and increase the activation of Caspase-3. The combinedinhibitory effect of curcumin and oxaliplatin on the proliferation of humangastric cancer cells BGC-823was more significant than curcumin andoxaliplatin alone, the combined apoptosis-inducing effect of curcumin andoxaliplatin on the human gastric cancer cells BGC-823was moresignificant than curcumin and oxaliplatin alone. |
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