The Expression Of VEGF-C, VEGFR-3 In The Squamous Carcinoma Of The Cervix And Their Relationship With Occurrence, Development And Metastasis

Tumor metastasis is one of important factors which influences prognosis of cancer of the cervix patients. Tumor metastasis depends on lymphangiogenesis and angiogenesis. VEGF-C is one of major member of VEGF family, which is able to bind and activity VEGFR-3 and VEGFR-2, participating in lymphangiogenesis, playing the important roles in lymphatic metastasis. VEGFR-3 belongs to VEGF PTK receptor family member and expresses lymphatic endothelial cell limitedly and is molecular marker of lymphatic endothelial cell. At present, VEGF-C expresses in many kinds of tumors such as carcinoma of prostate, ophageal carcinoma and gastric cancer and so on and its relationship with lymphatic metastasis, Scholars pay close attention. However, The research of the expression of VEGF-C and lymphangiogenesis of cancer of the cervix and lymphatic metastasis has few reporter in exterior and interior, Our research adoped immunohistochemical SP methods to observe the expression of normal mucosa of cervix, cervical intraepithelial neoplasia and the tissues of cancer of the cervix respectively and its relationship with lymphatic metastasis, Our research explores furtherly the mechanisms of occurrence, development and metastasis of uterine cervix carcinoma and provide rationale for early diagnosis, early treatment of uterine cervix cancer.Methods1. Using the technology of immunohistochemistry SP to detect the expression of VEGG-C and VEGFR-3 protein in the 33 cases of normal mucosa of uterine cervix, 63 cases of cervical intraepithelial neoplasia (CIN) (CIN I 16 cases, CIN II 22 cases, CINIII25 cases) tissues and 45 cases of squamous cell carcinoma of uterine cervix respectively.2. Statistical analysis: All the dates were analyzed by SPSS 11.0 statistical package, the count information calculated the positive rate, The comparison of positive rates uses the Chi-square; The relation of two variable are analyzed by the spearman level correlation analysis. The level of significant difference isα=0.05.Results1 .the positive expression rate of VEGF-C protein in normal mucosa of uterine cervix is 0%(0/33); The positive expression rate of VEGF-C protein in cervical intraepithelial neoplasia ( CIN I～II and CIN III ) tissues is 18.40%(7/38), 48.00%(12/25)); The positive expression rate of VEGF-C protein in the carcinoma tissues is 62.22%(28/45), the positive expression rate of VEGF-C protein in the carcinoma tissues is compared with that in the propotional adjacent tissues and normal mucosa tissues from each other, There is stastital significance( P <0.05).2. the positive expression rate of VEGFR-3 protein in normal mucosa of uterine cervix is 0%(0/33); The positive expression rate of VEGFR-3 protein in cervical intraepithelial neoplasia (CIN I～II and CIN III) tissues is 21.05%(8/38), 52.00%(13/25); The positive expression rate of VEGFR-3 protein in the carcinoma tissues is 68.89%(31/45), the positive expression rate of VEGFR-3 protein in the carcinoma tissues is compared with that in the propotional adjacent tissues and normal mucosa tissues from each other, There is stastital significance( P <0.05).3. The expression of VEGF-C is relative to regional nodal metastasis: in 28 cases patients with lymphatic metastasis, the positive expression of VEGF-C is higher than those non-lymphatic metastasis (P<0. 05); The expression of VEGFR-3 is relative to regional nodal metastasis: in 28 cases patients with lymphatic metastasis, the positive expression of VEGFR-3 is higher than those non-lymphatic metastasis (P<0.05 ).4. There is both statiscal significance for expression of VEGF-C, VEGFR-3 in different clinical stage( P>0. 05 ), with histological differentiated extend lowing, the expression rates of VEGF-C , VEGFR-3 both increased in turn, There is both statiscal significance (P<0.05) .5. The relativity between VEGF-C and VEGFR-3: The relativity of the expression of VEGF-C and VEGFR-3 was analysized by statistics, Results showedthat there is a positive correlation between both(P<0.01).Conclusions1. The positive rates of the expression of VEGF-C protein in squamous carcinoma tissues of uterine cervix, atypical hyperplasia tissues of the tumor-adjacent and normal mucosa tissues were high in turn, Therefore the comparison of the expression of VEGF-C protein among carcinoma tissues and adjacent atypical hyperplasia tissues , normal mucosa tissues has statistical signicance. It indicaed that VEGF-C participated in occurrence and development of squamous carcinoma of uterine cervix.2. The positive rates of the expression of VEGFR-3 protein in squamous carcinoma tissues of uterine cervix, atypical hyperplasia tissues of the tumor-adjacent and normal mucosa tissues were high in turn, Therefore the comparison of the expression of VEGFR-3 protein among carcinoma tissues and adjacent atypical hyperplasia tissues, normal mucosa tissues has statistical signicance. It indicaed that VEGFR-3 participated in occurrence and development of squamous carcinoma of uterine cervix.3. The expressions of VEGF-C , VEGFR-3 are not both related to clinical stage, While the expressions of VEGF-C , VEGFR-3 are not negatively related to histological stage, It indicated that they promoted the progression of cancer of the cervix.4. In patients with lymphatic metastasis, the positive expression of VEGF-C and VEGFR-3 was higher than those without lymphatic metastasis, It indicated that VEGF-C and VEGFR-3 may promoted lymphangiogenesis and accelerated infiltration and metastasis of cancer of the cervix.5. The relativity of the expression of VEGF-C and VEGFR-3 protein was analysized by statistics, Results showed relative and they played synergistic effect during the process of occurrence, development, infiltration and metastasis of squamous carcinoma of uterine cervix.