The Mobilization Of The Adenovirus Vector Encoding Hepatocyte Growth Factor Gene (Ad₅-HGF) To Hematopoietic Stem Cells In Peripheral Blood Of Patients With Coronary Heart Disease

Partâ… the mobilization of the adenovirus vector encoding Hepatocyte growth factor gene (Ad₅-HGF) to hematopoietic stem cells in peripheral blood of patients with coronary heart diseaseObjective: To investigate the mobilization to hematopoietic stem cells via noninfarct-relative artery therapy with the adenovirus vector encoding Hepatocyte growth factor gene (Ad₅-HGF) in patients with extensive coronary heart disease.Methods: 18 patients with cardiovascular classified into 2 groups: patients of therapy with the adenovirus vector encoding Hepatocyte growth factor (Ad₅-HGF)gene group (n=9) and patients with equal physiological saline as control group (n=9). Angioplasty and stent implantation was performed according to standard clinical practice by the femoral approach and Blood samples were drawn from each patient: baseline before PCI, 6 to 24 hours and 6days in all patients. The number of CD34+, CD38+, and CD117+ cells in peripheral blood was analyzed by flow cytometer. Results: The number of circulating CD34+ cells in Ad5-HGF gene treatment group was more than that in the control group after 6 hours of operation(P=0.021), There was no significant difference before PCI, 24 hours and 6 days between the Ad5-HGF gene treatment group and control group; The number of circulating CD117+ cells in Ad5-HGF gene treatment group was more than that in the control group after 6 days of operation(P=0.034), But there was no significant difference before 6 days between the two groups; The number ofcirculating CD38+ cells of two groups was no significant difference before PCI, 6 to 24 hours, and 6 days.Conclusion: Intracoronary administration of Ad₅-HGF and could shortly mobilized hematopoietic stem cells into peripheral blood, the mobilization of hematopoietic stem cells may be plays a critical role in the regulation of exotic HGF of angiogenesis, cell proliferation and regeneration myocardial tissue. Partâ…¡the effects of intracoronary and intramyocardial injection adenovirus vector encoding Hepatocyte growth factor gene (Ad₅-HGF) on hematopoietic stem cells in peripheral blood of patients with coronary heart diseaseObjective : To investigate the mobilization of intracoronary injection of adenovirus vector encoding Hepatocyte growth factor gene (Ad₅-HGF) compared with intramyocardial injection to patients with extensive coronary heart disease.Methods : 12 patients with cardiovascular disease were classified into 2 groups: patients of intracoronary injection the adenovirus vector encoding Hepatocyte growth factor (Ad₅-HGF) group (n=6); patients of intramyocardial injection the adenovirus vector encoding Hepatocyte growth factor (Ads-HGF) group (n=6). Angioplasty was performed according to standard clinical practice by the femoral approach. Blood samples were drawn from each patient before the operation, 6, 24 hours and 6 days respectively after operation in all patients. The number of CD34+ and CD117+ cells in peripheral blood was analyzed by flow cytometer.Results: The absolute number of CD34+cells was significantly higher in intracoronary injection group at 24h compared with intramyocardial injection group(P=O.042). There was a trend toward further increase after the operation, but the change did not reach statistical significance; The number of circulating CD117+ cells in intracoronary treatment group was significantly higher than that in intramyocardial injection group at 6h and 24h after the operation(P=0.018,P=0.030), Compared with before the opration the numbers of CD117+ was toward further increase, but the change did not reach statistical significance.Conclusion: Intracoronary administration of Ad₅-HGF compared with intramyocardial injection could obviously mobilized hematopoietic stem cells into peripheral blood, the mobilization of hematopoietic stem cells may play a critical role in the effects of exotic HGF on angiogenesis, cell proliferation and myocardial tissue regeneration...