Asessment Technic Of Cutaneous Absorption Of DanshinoneⅡA In Vivo Study

Danshen is chinese traditional medicine in common use, especially in externalusing. It have efficacy of Activating Blood Circulation Against Stasis, detumescenceacesodyne and incresing blood smoothing thenerves. The parts in Danshen which cantransdermal are fat-solubility substances such as Danshinone etc. and Danshinoneâ…¡Ais representation part.In china pharmacopoeia the content of Danshinoneâ…¡A is usedto control the Danshen quality.The paper study dermal penetration of Danshinoneâ…¡A using microdialysis invivo. We develop long membrane microdialysis probe which suit to experiment. Weembed probe into epidermis and spread Danshen unguent on skin then we collectsample interval 30 minute and use HPLC to analysis concentration of sample, later wecan draw the curve of concentration versus time of Danshinoneâ…¡A and calcute thetransdermal efficiency, analyse pharmacokinetics parameter.From the Danshinoneâ…¡A pharmacokinetics curve in infinity dose,it indicatesthat Danshinoneâ…¡A transdermal drug delivery accord Fick first diffuse law. The curvealso show Tlag time amount to2.646h and the dermal concentration of Danshinoneâ…¡Ado not decline after we move the medicine.The experiment also reveal that we can use microdialysis to collect subcutaneoussample from rat dermal and get steady result.