Clinical Study Of Dendritic Cell Combined With NP Project Chemotherapy For Non Small Cell Lung Cancer

Objective:To observe the clinical curative effect and side effect of the combined chemotherapy in non small cell lung cancer. To establish the specific immunological indexes of sensitized dendritic cell by observing the change of IFN-γ, IL-10 and IL-12 in the peripheral blood in non small. lung cancer before and after dendritic cell combined with NP project chemotherapy.Methods: Forty patients with non small lung cancer were randomly divided into chemotherapy group (control group) and dendtritic cell combined with chemotherapy group (observing group), sensitized matured DCs were derived from peripheral blood monocytes of the patients in observing group before chemotherpy which were loaded tumor lysate and cultivated in vitro, in the course of cultivation, the patients in observing group received Navelbine(NVB) plus Cisplatine(DDP), sensitized matured DCs were administered after chemotherapy, the patients in control group received the treatment of the same regimen without being administered sensitized matured DCs. The concentrations of IFN-γ, IL-10, IL-12in blood serum of patients with NSCLC were detected before and after treatment. The toxicity, clinical benefit response rate and KPS of patients with NSCLC were observed.Results: No statistical difference was found in clinical benefit response rate between two groups(P＞0.05). Compared with control group, bone marrow suppression was mild in obversing group(P＜0.05). In observing group, when sensitized matured DCs were administered, fever was a common side effect, serious side effects were not observed. The concentrations of IFN-γ, IL-10, IL-12in blood serum and KPS were not significant difference in two groups before treatment. In observing group, the concentrations of IFN-γ, IL-12 in blood serum and KPS were significantly increased, and the concentration of IL-10 was decreased after treatment. In control group, the concentrations of IFN-γ, IL-12 in blood serum and KPS were decreased, and the concentration of IL-10 was increased after chemotherapy, there was statistical difference between two groups.Conclusions: The response rate of biochmotherapy is the same as chemotherapy. Dendritic cell could decrease side effects of chemotherapy, change immunosuppression, enhance immune function and improve patients' quality of life.IFN-γ,IL-10 and IL-12 are the specific immunological indexes of sensitized dendritic cell therapy.