The Correlation With T-lymphocyte Subsets,HBeAg Level,ALT/AST And The Diseases With HBV Infection

Objective To investigate the change of the T-lymphocyte subsets,Hepatitis B e antigen(HBeAg) level, and alanine transminase/aspartate aminotransferase(ALT/AST) inperipheral blood of patients with Chronic Hepatitis B(CHB),Posthepatic Cirrhosis(PC)and Primary Hepatocelluar Carcinoma (PHC), and to explore their relationships with thediseases.Methods Peripheral blood was collected from 50 patiens with CHB,50 with PC, 50 withPHC and 30 healthy individuals. Flow cytometry was used to detect CD4~+ andCD8~+T-lymphocyte percentage (CD4~+T%,CD8~+T%), HBeAg level was detected byelectro-chemiluminnescence immunoassay(ECLIA) and ALT, AST were detected bykinetic method.Results The values of CD4~+T%,CD8~+T%,CD4~+/CD8~+,ALT/AST and HBeAg level inevery group were as follow:Normal controls group (39.14±6.24, 26.72±6.64,1.46±0.22,1.29±0.18, 0); CHB group (37.89±7.26, 28.52±7.02, 1.16±0.31, 1.52±0.80,542±382.1); PC group (30.16±6.24, 33.24±8.21, 0.91±0.38,0.69±0.25, 413±300.3);PHC group (31.27±6.82, 35.28±7.12, 0.89±0.41,0.68±0.38, 392±282.5). Compared withnormal controls group,the ALT/AST,CD4~+T% and CD4~+/CD8~+ all decreased andCD8~+T% increased in PC and PHC (P＜0.001, P＜0.01, P＜0.001, P＜0.01 ),as well as thosecompared with CHB group (P＜0.001, P＜0.01, P＜0.05, P＜0.01). The HBeAg positiverate in PC(34%,P＜0.05) and PHC(22%,P＜0.01) was significantly lower than it in CHB(60%)。No significant difference was found between PC group and PHC group. HBeAglevel was no statistical significance among the three groups.Moreover, there was nosignificant difference in HBeAg positive group and HBeAg negative group. ALT/ASTwas positive correlation with CD4~+/CD8~+(r=0.601, P＜0.05 ) and negative correlation withCD8~+T% (r=-0.562, P＜0.05).Conclusions There was turbulence in cellular immunity in patients with HBV infection.The decrease of CD4~+/CD8~+ and the serious injury of liver cells might be associated withHBV persistent infection.It is important to monitor the CD4~+/CD8~+ ratio and ALT/ASTratio for the diagnose and therapy of diseases with HBV infection.